Receptor tyrosine kinase (RTK)-initiated signaling mechanisms are major pathways critical for vascular development and angiogenesis, and mediate signals from angiogenic factors including FGF and VEGF. Our long-term goal is to elucidate the mechanisms by which angiogenic RTK signaling is regulated to direct normal development and homeostasis of the vasculature as a prerequisite to the development of therapeutic interventions of vascular defects and disease. Studies in Drosophila and mammalian cell culture systems have revealed that members of the Sprouty family of polypeptides are negative regulators of FGF signaling as well as other receptor tyrosine kinases (RTKs). During the previous funding period, we demonstrated that Spry2 inhibits FGF signaling at the level of Raf1, thus inhibiting ERK activation. Furthermore, we generated conditional transgenic mice for the tissue-specific expression of Spry1. Targeted expression of Spry1 to the vasculature in early development results in embryonic lethality and a loss of PECAM-positive cells. In addition, we have characterized a new feedback inhibitor of FGF signaling called Sef (similar expression as fgf) and show that Sef is expressed in endothelial cells and induces apoptosis when over expressed. We have shown that Sef physically associates with FGFR1 and inhibits FGFR1 tyrosine phosphorylation and all subsequent signaling downstream of FGFR1. The experiments proposed in this competitive renewal are aimed at elucidating the role of Spry family members in endothelial cell growth and differentiation in vivo. Additional experiments are aimed to determine whether Spry1 functionally interacts with other Spry family members and with Sef to fine tune angiogenic RTK signaling outputs. We hypothesize that Spry is a key regulator of vascular development and homeostasis in vivo, and the output of this regulation is determined by the particular proteins that Spry interacts with in a given cellular context. Therefore we propose the following specific aims: 1) to determine the functional interaction of Spry and Sef as a regulatory network that fine tunes angiogenic RTK- mediated signaling outputs such as ERK activation, 2) to use gain-of-function approaches to determine the role of Spry1 in endothelial cell proliferation and differentiation in transgenic mouse models, and 3) to use loss-of- function approaches to determine the role of Spry1 in endothelial cell proliferation and differentiation in transgenic and knockout mouse models. These studies will provide significant insight into feedback inhibition of RTK signaling via FGF and VEGF in endothelial cells with broad implications for other areas of biology including tumor formation.
Cardiovascular diseases are a major health problem worldwide. Receptor tyrosine kinases (RTKs) play a major role in vascular homeostasis and disease and new pathways are emerging that control the activity of these RTKs. Our goal is to study these pathways at the cellular, molecular and genetic level to increase our knowledge to enable the development new treatments for cardiovascular disease.
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