Fetal sheep will be exposed to intraamniotic endotoxin and the minimal effective dose determined as well as the effects of prolonged low dose exposure. Subsequently, the route of the effects of endotoxin will be evaluated by targeting the stimulus to the amniotic cavity, the systemic circulation, the GI tract, or the lungs. All lambs exposed in utero will be delivered preterm and evaluated for indicators of amniotic space inflammation, lung function, and lung biochemical indicators of maturation. Candidate mediators, identified by evaluating the fetal responses to endotoxin, will be tested for their ability to induce fetal responses and lung maturation.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL065397-03
Application #
6527359
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Program Officer
Berberich, Mary Anne
Project Start
2000-09-01
Project End
2004-08-31
Budget Start
2002-09-01
Budget End
2003-08-31
Support Year
3
Fiscal Year
2002
Total Cost
$323,955
Indirect Cost
Name
Cincinnati Children's Hospital Medical Center
Department
Type
DUNS #
071284913
City
Cincinnati
State
OH
Country
United States
Zip Code
45229
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