The goal is to postpone circulatory collapse for at least 4 hrs (post hemorrhage) in severe hemorrhagic shock. This extension of the """"""""Golden Hour"""""""" will be attempted in anesthetized pigs by administration of a novel, logistically very convenient treatment consisting in i.v. infusion of small doses (< 1.0ml/kg) of dodecafluoropentane emulsion (DDFPe), which at body temperature generates microbubbles that transport oxygen. A shock model which is fatal in pigs (controls) in 4 hrs will be treated at different points within this time interval. The ability of plasma expander administered thereafter to allow long-term (weeks) survival will be tested. A multitude of physiological and biochemical parameters will be monitored and organ histology studied. Markers of circulatory collapse will in particular be: subnormal tissue oxygen tensions in brain, liver, kidney and skeletal muscle, reductions in heart rate, deep body temperature and oxygen consumption, increasing lactacidemia and falling base excess and diuresis. Indicators of health during long-term survival include liver and kidney function tests. The project is expected to bring the DDFPe treatment to a state of scientific underpinning and applicability in the animal model which lays a solid foundation for pursuing FDA approval for human use.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL076158-01
Application #
6754146
Study Section
Special Emphasis Panel (ZHL1-CSR-I (F1))
Program Officer
Lathrop, David A
Project Start
2004-01-01
Project End
2007-12-31
Budget Start
2004-01-01
Budget End
2004-12-31
Support Year
1
Fiscal Year
2004
Total Cost
$392,500
Indirect Cost
Name
State University of New York at Buffalo
Department
Physiology
Type
Schools of Medicine
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260