The importance of coronary atherosclerosis and its clinical manifestations cannot be overstated;in this country, half a million people die from acute myocardial infarction every year and an equal number of patients survive with substantial morbidity. Coronary atherosclerosis is an inflammatory process governed by dysregulated endothelium, leukocytes, macrophages, and smooth muscle cells. These cells conspire to form lesions within the artery wall, effectuate plaque progression or regression, and play a critical role in precipitating thrombosis, which can block blood flow to the heart. Progress towards improving the understanding, diagnosis, and treatment of coronary artery disease has been slowed by our inability to observe these cells in the coronary arteries of living human patients. Our laboratory has been on the forefront of pushing the resolution limits of coronary imaging in vivo. In the parent R01, we developed an intracoronary imaging technology, optical frequency domain imaging (OFDI), that makes it practical to obtain 10-?m resolution, three-dimensional images of the coronary wall in the clinical setting. Now that OFDI has been transferred to the commercial sector, we will focus our efforts on developing the next generation coronary imaging technology, one that will provide images at a resolution of 1-?m. This advance will enable the investigation of macromolecules and cells involved in the pathophysiology of CAD and will allow clinicians to diagnose and treat this disease based on cellular information obtained from their patients'coronary arteries. The research strategy of this project parallels that of our previous development and translation of intracoronary OFDI. First, new ?OCT technology will be designed and fabricated, focusing on solving catheter and system- level challenges. Alongside this development process, we will validate the accuracy of ?OCT ex vivo for identifying relevant cellular and subcellular features of the coronary wall, using histopathology as a gold standard. The safety, feasibility, and efficacy of ?OCT will then be evaluated in 25 patients undergoing coronary intervention. !

Public Health Relevance

Coronary artery disease, the number one cause of death in the US, is caused by cells in the walls of the arteries that supply blood to the heart. These cells are too small to be visualized using modern medical imaging technologies. By providing a new imaging method that will allow scientists and physicians to see these cells in patients, this research will change how we study, diagnose, and treat this disease.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Project (R01)
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Biomedical Imaging Technology Study Section (BMIT)
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Fleg, Jerome
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Massachusetts General Hospital
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Yin, Biwei; Hyun, Chulho; Gardecki, Joseph A et al. (2017) Extended depth of focus for coherence-based cellular imaging. Optica 4:959-965
Yin, Biwei; Chu, Kengyeh K; Liang, Chia-Pin et al. (2016) ?OCT imaging using depth of focus extension by self-imaging wavefront division in a common-path fiber optic probe. Opt Express 24:5555-5564
Suter, Melissa J; Kashiwagi, Manabu; Gallagher, Kevin A et al. (2015) Optimizing flushing parameters in intracoronary optical coherence tomography: an in vivo swine study. Int J Cardiovasc Imaging 31:1097-106
Conti de Freitas, Luiz C; Phelan, Eimear; Liu, Linbo et al. (2014) Optical coherence tomography imaging during thyroid and parathyroid surgery: a novel system of tissue identification and differentiation to obviate tissue resection and frozen section. Head Neck 36:1329-34
Kashiwagi, Manabu; Liu, Linbo; Chu, Kengyeh K et al. (2014) Feasibility of the assessment of cholesterol crystals in human macrophages using micro optical coherence tomography. PLoS One 9:e102669
Liu, Linbo; Chu, Kengyeh K; Houser, Grace H et al. (2013) Method for quantitative study of airway functional microanatomy using micro-optical coherence tomography. PLoS One 8:e54473
Fard, Ali M; Vacas-Jacques, Paulino; Hamidi, Ehsan et al. (2013) Optical coherence tomography--near infrared spectroscopy system and catheter for intravascular imaging. Opt Express 21:30849-58
Hara, Tetsuya; Bhayana, Brijesh; Thompson, Brian et al. (2012) Molecular imaging of fibrin deposition in deep vein thrombosis using fibrin-targeted near-infrared fluorescence. JACC Cardiovasc Imaging 5:607-15
Ha, Jinyong; Yoo, Hongki; Tearney, Guillermo J et al. (2012) Compensation of motion artifacts in intracoronary optical frequency domain imaging and optical coherence tomography. Int J Cardiovasc Imaging 28:1299-304
Coron, E; Auksorius, E; Pieretti, A et al. (2012) Full-field optical coherence microscopy is a novel technique for imaging enteric ganglia in the gastrointestinal tract. Neurogastroenterol Motil 24:e611-21

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