The objective of the proposed project is to develop a real time high frequency ultrasound imaging system with a frame rate at a minimum of 180 Hz for cardiac applications in small animals. Ultrasound imaging is a well established technology for cardiac imaging on humans. For cardiac imaging in small animals whose hearts beat at a rate higher than 400 beats/minute, the spatial and temporal resolutions of current clinical ultrasonic scanners are far from ideal and simply inadequate for such applications. Furthermore currently there are no commercial high frequency ultrasonic scanners developed for small animal imaging and eye imaging in humans that are capable of a frame rate higher than 30 Hz over a large field of view. Under the support of this grant a device which a mechanical sector scanner with a single element transducer capable of imaging the heart of a small animal at a frequency from 30 to 50 MHz at a frame rate at a minimum of 130 Hz has been designed, fabricated, and tested. Initial experiments performed on mice and zebrafish hearts. In the next grant period, the performance of the scanner will be further improved by increasing the frequency and frame rate and implementing coded excitation to increase the depth of penetration. Miniature light weight annular arrays which allow dynamic focusing will be developed to replace single element transducers for improved spatial resolution taking advantage of an annular array based digital imaging system being developed at the USC Transducer Resource. The servo-motor based mechanical probe will be developed in collaboration with Capistrano Lab. Potential applications of this device in quantitative measurements of cardiac anatomy and physiological functions in mice and zebrafish will be explored in collaborations with investigators from USC Children's Hospital and Medical School.

Public Health Relevance

Project Narrative Small animals, including mice, rats, zebrafish, etc., have emerged as ideal models for the study of human diseases and drug development. Therefore, as the demand for the studies of small animals increases, so does the need for methods to accurately monitor physiological and functional activities on a small scale. The high frequency ultrasonic system will offer a multitude of opportunities for biomedical research.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Project (R01)
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Study Section
Biomedical Imaging Technology Study Section (BMIT)
Program Officer
Buxton, Denis B
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University of Southern California
Biomedical Engineering
Schools of Engineering
Los Angeles
United States
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Kang, Bong Jin; Park, Jinhyoung; Kim, Jieun et al. (2015) High-frequency dual mode pulsed wave Doppler imaging for monitoring the functional regeneration of adult zebrafish hearts. J R Soc Interface 12:
Park, Jinhyoung; Li, Xiang; Zhou, Qifa et al. (2013) Combined chirp coded tissue harmonic and fundamental ultrasound imaging for intravascular ultrasound: 20-60 MHz phantom and ex vivo results. Ultrasonics 53:369-76
Park, Jinhyoung; Cummins, Thomas M; Harrison, Michael et al. (2013) High frequency photoacoustic imaging for in vivo visualizing blood flow of zebrafish heart. Opt Express 21:14636-42
Qiu, Weibao; Yu, Yanyan; Chabok, Hamid Reza et al. (2013) A flexible annular-array imaging platform for micro-ultrasound. IEEE Trans Ultrason Ferroelectr Freq Control 60:178-86
Park, Jinhyoung; Huang, Ying; Chen, Ruimin et al. (2013) Pulse inversion chirp coded tissue harmonic imaging (PI-CTHI) of Zebrafish heart using high frame rate ultrasound biomicroscopy. Ann Biomed Eng 41:41-52
Zhou, Xiaowei; Sun, Lei; Yu, Yanyan et al. (2013) Ultrasound bio-microscopic image segmentation for evaluation of zebrafish cardiac function. IEEE Trans Ultrason Ferroelectr Freq Control 60:718-26
Liu, Changgeng; Djuth, Frank; Li, Xiang et al. (2012) Micromachined high frequency PMN-PT/epoxy 1-3 composite ultrasonic annular array. Ultrasonics 52:497-502
Hwang, Jae Youn; Park, Jinhyoung; Kang, Bong Jin et al. (2012) Multimodality imaging in vivo for preclinical assessment of tumor-targeted doxorubicin nanoparticles. PLoS One 7:e34463
Park, Jinhyoung; Hu, Changhong; Li, Xiang et al. (2012) Wideband linear power amplifier for high-frequency ultrasonic coded excitation imaging. IEEE Trans Ultrason Ferroelectr Freq Control 59:825-32
Park, Jinhyoung; Lee, Jungwoo; Lau, Sien Ting et al. (2012) Acoustic radiation force impulse (ARFI) imaging of zebrafish embryo by high-frequency coded excitation sequence. Ann Biomed Eng 40:907-15

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