Platelet activation plays a major role in hemostasis and thrombosis. Platelet agonists cause shape change, fibrinogen receptor activation, dense granule release, and thromboxane A2 (TXA2) generation, leading to the activation of other platelets. The mechanisms regulating these platelet physiological events have not been completely understood. All the platelet agonists, either directly or indirectly, depend on G protein pathways to cause platelet activation. We propose to further understand the downstream events in the G protein pathways and their second messengers in platelet activation using complementary biochemical, pharmacological, and gene knockout approaches. ADP and thrombin differ in their ability to activate platelets. ADP fails to cause dense granule release in aspirin-treated platelets, whereas thrombin can. ADP depends on integrin signaling to activate phospholipase A2, whereas thrombin does not. Whereas both these agonists activate Gq-phospholipase C pathways, only thrombin stimulates G12/13 pathways. This grant application is built upon our recent studies demonstrating important roles for a) G12/13 pathways in platelet fibrinogen receptor activation, b) protein kinase C delta isoform in thromboxane generation, and c) Gi pathways in Akt phosphorylation in platelets. We will test the hypothesis that G12/13 pathways contribute to dense granule release, TXA2 generation, and Akt phosphorylation and activation, using pharmacological approaches complemented with platelets from Galpha12 and Galpha13 gene knockout mice and constitutively active Galpha12 and Galpha13 transgenic mice. We will evaluate the relative contributions of Gq/PLC pathways and G12/13 pathways to agonist-induced dense granule release, TXA2 generation, and Akt phosphorylation. We also hypothesize that HAX-1, HS-1, and Src family kinases are activated downstream of G12/13 pathways, which play an important role in platelet activation. We will delineate some of these signaling molecules downstream of the G12/13 pathways, as this pathway is the least understood in platelets. Finally, we will evaluate the functional role of HS1 in platelets using mice-deficient in HS1 in ex vivo platelet functional studies and in vivo thrombosis models. We have strong preliminary data supporting each of the above specific aims. These studies will enhance our understanding of the signaling pathways and their role in platelet activation, and might identify potential newer targets for the treatment of thrombosis.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL081322-05
Application #
7797553
Study Section
Special Emphasis Panel (ZRG1-HEME-A (02))
Program Officer
Sarkar, Rita
Project Start
2006-04-01
Project End
2012-03-31
Budget Start
2010-04-01
Budget End
2012-03-31
Support Year
5
Fiscal Year
2010
Total Cost
$529,845
Indirect Cost
Name
Temple University
Department
Physiology
Type
Schools of Medicine
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
Bhavanasi, Dheeraj; Kostyak, John C; Swindle, John et al. (2015) CGX1037 is a novel PKC isoform delta selective inhibitor in platelets. Platelets 26:2-9
Rico, Mario C; Rough, James J; Manns, Joanne M et al. (2012) Assembly of the prothrombinase complex on the surface of human foreskin fibroblasts: Implications for connective tissue growth factor. Thromb Res 129:801-6
Getz, T M; Mayanglambam, A; Daniel, J L et al. (2011) Go6976 abrogates GPVI-mediated platelet functional responses in human platelets through inhibition of Syk. J Thromb Haemost 9:608-10
Bhavaraju, Kamala; Lakhani, Parth R; Dorsam, Robert T et al. (2011) G(12/13) signaling pathways substitute for integrin ?IIb?3-signaling for thromboxane generation in platelets. PLoS One 6:e16586
Kim, Soochong; Kunapuli, Satya P (2011) Negative regulation of Gq-mediated pathways in platelets by G(12/13) pathways through Fyn kinase. J Biol Chem 286:24170-9
Bhavanasi, Dheeraj; Kim, Soochong; Goldfinger, Lawrence E et al. (2011) Protein kinase C? mediates the activation of protein kinase D2 in platelets. Biochem Pharmacol 82:720-7
Buitrago, Lorena; Langdon, Wallace Y; Sanjay, Archana et al. (2011) Tyrosine phosphorylated c-Cbl regulates platelet functional responses mediated by outside-in signaling. Blood 118:5631-40
Mayanglambam, Azad; Bhavanasi, Dheeraj; Vijayan, K Vinod et al. (2011) Differential dephosphorylation of the protein kinase C-zeta (PKC?) in an integrin ?IIb?3-dependent manner in platelets. Biochem Pharmacol 82:505-13
Nagy Jr, B; Jin, J; Ashby, B et al. (2011) Contribution of the P2Y12 receptor-mediated pathway to platelet hyperreactivity in hypercholesterolemia. J Thromb Haemost 9:810-9
Bynagari-Settipalli, Yamini S; Chari, Ramya; Kilpatrick, Laurie et al. (2010) Protein kinase C - possible therapeutic target to treat cardiovascular diseases. Cardiovasc Hematol Disord Drug Targets 10:292-308

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