Ischemic heart disease (IHD) is a major epidemic throughout the developed world, yet the molecular mechanisms that lead to myocardial cell death in this disorder are not completely understood. Evidence indicates that the mitochondria play an important role in regulating ischemic-induced cellular injury. We previously demonstrated that overexpression of a mitochondrial protein, the mitochondrial ATP-binding cassette protein-1 (mABC1) protects against oxidant induced cell death, while its downregulation causes an increase in cell death at baseline. The primary function of mABC1 is unknown, thus it is unclear how this protein exerts protection against oxidant stress. The yeast homolog of mABC1, Mdl1p, has also been shown to exert protection against oxidant-induced cellular injury and plays a role in mitochondrial iron homeostasis. Intracellular iron accumulation can be a source of oxidative stress, which is thought to be the underlying mechanism for the protective effects of Mdl1p. In this proposal, we will follow up on our key observation that mABC1 is protective against oxidant injury and propose to elucidate the underlying mechanism for this process. Furthermore, we will attempt to extend our studies to intact animals and determine whether mABC1 can also protect against ischemic injury in the heart. Our main hypothesis is that mABC1 plays a role in the maintenance of the mitochondrial iron homeostasis and that it protects against ischemic damage in the heart. In order to test this hypothesis, we propose three interrelated specific aims.
In Aim 1, we will determine whether mABC1 plays a role in the maintenance of mitochondrial iron homeostasis and generation of intracellular reactive oxygen species. The levels of mABC1 will be modulated in neonatal rat cardiomyocytes (NRCM) using various molecular biology techniques, followed by measurement of the mitochondria iron and intracellular ROS levels.
In Aim 2, we will determine whether the protective effects of mABC1 are through inhibition of the mitochondrial permeability transition pore (mPTP). This channel is believed to be a pivotal player in oxidant-induced cell death. By altering the levels of mABC1 in NRCM, we will study the effect of mABC1 on cell viability and mPTP opening.
In Aim 3, we will make transgenic mice that overexpress mABC1, followed by induction of ischemia/reperfusion (I/R). The effects of mABC1 overexpression on l/R-induced cell death will then be examined by various techniques. These lines of investigation promise to advance our knowledge of the molecular mechanisms of myocardial cell death in ischemic heart disease. Furthermore, successful completion of our studies will have potential impact on our understanding of the role of mitochondria in cardiovascular disease, and may open new avenues for the treatment of IHD.
| Rines, Amy K; Chang, Hsiang-Chun; Wu, Rongxue et al. (2017) Snf1-related kinase improves cardiac mitochondrial efficiency and decreases mitochondrial uncoupling. Nat Commun 8:14095 |
| Chang, Hsiang-Chun; Bayeva, Marina; Taiwo, Babafemi et al. (2015) Short communication: high cellular iron levels are associated with increased HIV infection and replication. AIDS Res Hum Retroviruses 31:305-12 |
| Bayeva, Marina; Sawicki, Konrad Teodor; Butler, Javed et al. (2014) Molecular and cellular basis of viable dysfunctional myocardium. Circ Heart Fail 7:680-91 |
| Ichikawa, Yoshihiko; Ghanefar, Mohsen; Bayeva, Marina et al. (2014) Cardiotoxicity of doxorubicin is mediated through mitochondrial iron accumulation. J Clin Invest 124:617-30 |
| Rines, Amy K; Ardehali, Hossein (2013) Transition metals and mitochondrial metabolism in the heart. J Mol Cell Cardiol 55:50-7 |
| Bayeva, Marina; Khechaduri, Arineh; Wu, Rongxue et al. (2013) ATP-binding cassette B10 regulates early steps of heme synthesis. Circ Res 113:279-87 |
| Khechaduri, Arineh; Bayeva, Marina; Chang, Hsiang-Chun et al. (2013) Heme levels are increased in human failing hearts. J Am Coll Cardiol 61:1884-93 |
| Bayeva, Marina; Chang, Hsiang-Chun; Wu, Rongxue et al. (2013) When less is more: novel mechanisms of iron conservation. Trends Endocrinol Metab 24:569-77 |
| Marcovina, Santica M; Sirtori, Cesare; Peracino, Andrea et al. (2013) Translating the basic knowledge of mitochondrial functions to metabolic therapy: role of L-carnitine. Transl Res 161:73-84 |
| Bayeva, Marina; Gheorghiade, Mihai; Ardehali, Hossein (2013) Mitochondria as a therapeutic target in heart failure. J Am Coll Cardiol 61:599-610 |
Showing the most recent 10 out of 24 publications
