Recent studies from our laboratory and others suggest the existence of a complex cross- regulation of energy substrate metabolism between heart and periphery, mediated by peptides/proteins that add to insulin, glucagon or other classical regulators. Among the potential candidate mediators, particular attention deserve muscle-released, hormone-like factors collectively named myokines. One of the emerging members is follistatin-like protein 1 (Fstl1), which is produced also by the heart, as first showed by the Walsh laboratory, and can be therefore ascribed to the myokine subgroup named cardiokines. High circulating Fstl1 is a reliable marker of heart failure (HF), while, if acutely overexpressed, Fstl1 can protect the heart against ischemia-reperfusion injury. However, while the molecular biology and even the therapeutic potential of Fstl1 are being progressively elucidated and defined, virtually nothing is known yet about the integrative physiology and pathophysiology of this hormone. In particular, no studies have explored the potential role of Fstl1 as a metabolic modulator. Preliminary studies from Recchia and Walsh labs indicate that Fstl1 overexpression or infusion alters cardiac and systemic metabolism in mice and dogs. Therefore the present project will test the hypothesis that the relative fractions of Fstl1 produce by heart and skeletal muscle vary in response to physiological and pathological conditions and contribute proportionally to the autocrine and the reciprocal endocrine regulation of cardiac and systemic energy metabolism.
Three specific aims will be pursued combining studies in dog and mouse models.
Specific aim 1 will determine overall turnover and relative rate of Fstl1 production by cardiac and skeletal muscle at baseline and in response to exercise or to moderate and severe HF. These studies will be performed in chronically instrumented dogs under resting conditions and during exercise or the development of tachypacing-induced HF.
Specific aim 2 will test whether Fstl1 modulates cardiac and systemic oxygen and energy substrate consumption under physiological conditions and in HF. These studies will be performed in mouse models of cardiac or skeletal muscle- selective Fstl1 loss of function and in chronically instrumented dogs. The underlying molecular changes and mechanisms will be determined in tissue samples, ex vivo, as well as in cultured cardiomyocytes and skeletal myofibers exposed to Fstl1.
Specific aim 3 will test whether sustained, selective enhancement of cardiac Fstl1 synthesis can attenuate myocardial and/or systemic metabolic alterations and exert therapeutic effects in HF. These studies will be performed in cardio-specific Fstl1 knockout mice subjected to aortic constriction and in dogs with tachypacing-induced HF and subjected to cardiac gene transfer of Fstl1 carried by adeno- associated viral vectors. Combined studies in mice and dogs may lead to the translation of novel findings into a new biological therapy for HF.

Public Health Relevance

Heart and skeletal muscle should no longer be considered only as contracting organs deputed to perform mechanical work. They also release in the circulation a number of small proteins named cardiokines (from the heart) and myokines (from skeletal muscles), which function as hormones, i.e. messengers utilized to control the function other distant organs. In the present project we hypothesize that one of these muscle hormones, named follistatin-like protein 1 (Fstl1), is released by skeletal muscle to modulate oxygen and foodstuff consumption by the heart and, vice versa, the heart releases Fstl1 to regulate skeletal muscle metabolism. In other words, we hypothesize the existence of a metabolic crosstalk between heart and skeletal muscles. Of note, the concentration of Fstl1 increases in patients with heart failure. Our hypothesis will be tested by performing experiments both in mouse and in dog models. These two animal models offer complementary advantages for our research, therefore the integration of mouse and dog studies will lead to a much deeper understanding of the role of Fstl1 under normal and pathological conditions. Moreover, since the dog is a clinically-relevant animal model, our studies may lead to the relatively rapid translation of novel findings into a new therapy for heart failure, based on the use of Fstl1.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL129120-01A1
Application #
9113430
Study Section
Myocardial Ischemia and Metabolism Study Section (MIM)
Program Officer
Adhikari, Bishow B
Project Start
2016-04-06
Project End
2020-03-31
Budget Start
2016-04-06
Budget End
2017-03-31
Support Year
1
Fiscal Year
2016
Total Cost
Indirect Cost
Name
Temple University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
Lee, Kwangwon; Haddad, Andrew; Osme, Abdullah et al. (2018) Hepatic Mitochondrial Defects in a Nonalcoholic Fatty Liver Disease Mouse Model Are Associated with Increased Degradation of Oxidative Phosphorylation Subunits. Mol Cell Proteomics 17:2371-2386
McCullough, Arthur; Previs, Stephen; Kasumov, Takhar (2018) Stable isotope-based flux studies in nonalcoholic fatty liver disease. Pharmacol Ther 181:22-33
Gabisonia, Khatia; Recchia, Fabio A (2018) Gene Therapy for Heart Failure: New Perspectives. Curr Heart Fail Rep 15:340-349
Maruyama, Sonomi; Wu, Chia-Ling; Yoshida, Sumiko et al. (2018) Relaxin Family Member Insulin-Like Peptide 6 Ameliorates Cardiac Fibrosis and Prevents Cardiac Remodeling in Murine Heart Failure Models. J Am Heart Assoc 7:
Lindsey, Merry L; Bolli, Roberto; Canty Jr, John M et al. (2018) Guidelines for experimental models of myocardial ischemia and infarction. Am J Physiol Heart Circ Physiol 314:H812-H838
Seki, Mitsuru; Powers, Jeffery C; Maruyama, Sonomi et al. (2018) Acute and Chronic Increases of Circulating FSTL1 Normalize Energy Substrate Metabolism in Pacing-Induced Heart Failure. Circ Heart Fail 11:e004486