This proposal describes an Alzheimer's focused supplement to an R01 studying host control mechanisms against Klebsiella pneumoniae infection. The number of people living with Alzheimer's dementia in the United States is estimated at 5.8 million in 2020, and this number is expected to double by 2040. The role of infection in Alzheimer's dementia pathogenesis remains unclear, however, survivors of sepsis (severe infections) experience new cognitive deficits that persist for months to years after the inciting insult. The mechanisms underlying cognitive deficits after sepsis are unclear. In this proposal, we plan to study the role of the scavenger receptor CD36 in mediating cognitive decline and Alzheimer's dementia pathogenesis in septic mouse models. Our prior studies have focused on the host response following intrapulmonary Klebsiella pneumoniae infection where CD36 improves macrophage phagocytic function, reduces bacterial burden, and increases survival. In contrast to its beneficial effects in the host response, CD36 may be pathogenic in Alzheimer's dementia. The binding of CD36 to amyloid beta protein induces neuroinflammation and microglial cell activation leading to cognitive deficits in preclinical models. Furthermore, CD36 is increased in the brains of patient's with Alzheimer's dementia compared to age matched controls, and CD36 genetic polymorphisms are associated with increased risk of Alzheimer's disease. We hypothesize that while CD36 is beneficial in the host response to infection, CD36 activation during sepsis induces persistent neuroinflammation and contributes to cognitive decline thereby accelerating time to dementia, particularly in the setting of pre-existing amyloid beta deposits. We will test this hypothesis in an antibiotic-treated mouse model of long-term survival from intrapulmonary Klebsiella pneumoniae infection. We will examine the role of CD36 on microglial activation, cytokine and chemokine expression, amyloid deposition, reactive oxygen species generation, and development of cognitive deficits following sepsis using male and female C57BL/6J and CD36 -/- mice strains. In addition, we will perform studies in aged mice and in mice with an accelerated Alzheimer's dementia phenotype (AppNL-G-F) to model the effects of sepsis on older patients and on patients with predisposition to cognitive deficits. This proposal will be conducted by a multidisciplinary team at the University of Pittsburgh with expertise in host response to infection, cognitive deficits after sepsis, and Alzheimer's dementia, and will provide the foundation for future research focused on understanding relationships between infection and dementia, and preventing cognitive decline.
The global burden of Alzheimer's disease, the most common form of dementia, is rising underscoring a need to understand modifiable risk factors. The role of infection in Alzheimer's pathogenesis is unclear, however, survivors of severe infections experience new cognitive deficits that can persist for months to years after the inciting episode and may result in an accelerated time to dementia. In these proposed studies, we seek to understand the role of CD36, a scavenger receptor important in the host response to bacterial infection, in mediating neuroinflammation following infection with Klebsiella pneumoniae in preclinical mouse models with a focus on aged mice and mice with a predisposition to amyloid beta deposition.
| Ryu, Hyunryul; Choi, Kyungyong; Qu, Yanyan et al. (2018) Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics. J Vis Exp : |
