Abstract

The objective of the research proposed in this application is to generate basic data relating to the control of spinal cord motor processes during active sleep in the adult cat. The animal model which will be employed to achieve this objective is the acute decerebrate cat in which atonia of the peripheral musculature is induced by the microinjection of carbachol, a cholinergic agonist, into the pontine reticular formation (the nucleus pontis oralis). Intracellular recordings will be obtained from spinal cord motoneurons, spinal cord interneurons that provide a monosynaptic inhibitory input to spinal cord motoneurons, and from neurons of the medullary nucleus reticularis gigantocellularis; the neurotransmitters modulating the excitability of these cells will be examined by recording intracellularly from them at the same time that neurotransmitter antagonists and agonists are ejected juxtacellularly. The proposed studies will (1) provide a description of the cholinergically activated mechanisms and neurotransmitters that produce atonia of the somatic musculature, and (2) establish new techniques and an experimental paradigm that combines the strategic advantages of the acute preparation with a pharmacologically induced pattern of motor control that appears identical to that which occurs spontaneously during active sleep in the intact cat.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Project (R01)
Project #
5R01MH043362-02
Application #
3382831
Study Section
(SRCM)
Project Start
1988-04-01
Project End
1991-03-31
Budget Start
1989-04-01
Budget End
1990-03-31
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Xi, M; Fung, S J; Yamuy, J et al. (2015) Interactions between hypocretinergic and GABAergic systems in the control of activity of neurons in the cat pontine reticular formation. Neuroscience 298:190-9
Pose, I; Sampogna, S; Chase, M H et al. (2011) Nitrergic ventro-medial medullary neurons activated during cholinergically induced active (rapid eye movement) sleep in the cat. Neuroscience 172:246-55
Yamuy, Jack; Fung, Simon J; Xi, Mingchu et al. (2010) State-dependent control of lumbar motoneurons by the hypocretinergic system. Exp Neurol 221:335-45
Engelhardt, John K; Silveira, Valentina; Morales, Francisco R et al. (2010) Serotoninergic control of glycinergic inhibitory postsynaptic currents in rat hypoglossal motoneurons. Brain Res 1345:1-8
Xi, Mingchu; Chase, Michael H (2010) The injection of hypocretin-1 into the nucleus pontis oralis induces either active sleep or wakefulness depending on the behavioral state when it is administered. Sleep 33:1236-43
Vanini, G; Torterolo, P; McGregor, R et al. (2007) GABAergic processes in the mesencephalic tegmentum modulate the occurrence of active (rapid eye movement) sleep in guinea pigs. Neuroscience 145:1157-67
Morales, F R; Sampogna, S; Rampon, C et al. (2006) Brainstem glycinergic neurons and their activation during active (rapid eye movement) sleep in the cat. Neuroscience 142:37-47
Xi, M-C; Chase, M H (2006) Neuronal mechanisms of active (rapid eye movement) sleep induced by microinjections of hypocretin into the nucleus pontis oralis of the cat. Neuroscience 140:335-42
Torterolo, Pablo; Sampogna, Sharon; Morales, Francisco R et al. (2006) MCH-containing neurons in the hypothalamus of the cat: searching for a role in the control of sleep and wakefulness. Brain Res 1119:101-14
Zhang, Jian-Hua; Sampogna, Sharon; Morales, Francisco R et al. (2005) Age-related ultrastructural changes in hypocretinergic terminals in the brainstem and spinal cord of cats. Neurosci Lett 373:171-4

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