Neuropeptide Y (NPY) receptors in the paraventricular nucleus of the hypothalamus (PVN) process information from NPY afferents relaying sensory, cardiovascular and endocrine information from the brainstem and hypothalamus. Any perturbation in this system, such as that perceived during stressful conditions, will alter the activity of the PVN. Y1 and Y5 receptor levels in the PVN are in part responsible for fine tuning these inputs and producing an efferent signal that will affect corticotropin releasing factor (CRF) secretion, cardiovascular and sympathetic tone. The long- range objective of this proposal is to examine the anatomical distribution and regulation of Y1 and Y5 receptor subtypes within the PVN as they relate to the regulation of stress hormone secretion. The hypothesis that Y1 and Y5 receptors are present on neuroendocrine neurons that regulate CRF and adrenocorticotropin (ACTH) secretion will be assessed. In addition, we will test the hypotheses that Y1 and Y5 receptor expressing neurons are activated during stressful conditions and that NPY regulates the expression of its own receptors. The following specific aims will be tested: 1. Determine whether NPY neurons projecting to the PVN and Y1 and Y5 receptor-containing neurons are activated during stress: 2. Determine the ultrastructural localization of NPY Y1 and Y5 receptors within the PVN: 3. Identify the phenotype and projections of hypothalamic Y1 and Y5 receptor immunoreactive neurons in the PVN: 4. Examine the regulation and processing of Y1 and Y5 receptors by elevated levels of NPY. This will be accomplished by using a combination of anatomical tracing methods combined with single and double label immunocytochemistry at the light and electron microscope levels to determine the projections and phenotype of Y1 and Y5 receptive neurons in the PVN. Different stressors (physical, psychological and nutritional) and chronic administration of NPY will be used to test the responsiveness and regulation of Y1 and Y5 receptors to these challenges. These studies will provide significant information on the distribution and neuroanatomy of Y1 and Y5 receptor expressing cells in the PVN and their potential role in integrating stress responses in the PVN. The identification of NPY receptors on neural pathways and hypothalamic neuropeptide neurons will provide important information regarding the regulation and integration of stress hormone secretion and food intake. Dysregulation of these systems may be implicated in the development of stress related illness such as depression and eating disorders.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Project (R01)
Project #
5R01MH062621-05
Application #
7061657
Study Section
Special Emphasis Panel (ZRG1-IFCN-2 (01))
Program Officer
Desmond, Nancy L
Project Start
2002-09-10
Project End
2008-04-30
Budget Start
2006-05-10
Budget End
2008-04-30
Support Year
5
Fiscal Year
2006
Total Cost
$228,501
Indirect Cost
Name
Rosalind Franklin University
Department
Physiology
Type
Schools of Medicine
DUNS #
069501252
City
North Chicago
State
IL
Country
United States
Zip Code
60064
Leitermann, Randy J; Rostkowski, Amanda B; Urban, Janice H (2016) Neuropeptide Y input to the rat basolateral amygdala complex and modulation by conditioned fear. J Comp Neurol 524:2418-39
Rostkowski, Amanda B; Leitermann, Randy J; Urban, Janice H (2013) Differential activation of neuronal cell types in the basolateral amygdala by corticotropin releasing factor. Neuropeptides 47:273-80
Leitermann, Randy J; Sajdyk, Tammy J; Urban, Janice H (2012) Cell-specific expression of calcineurin immunoreactivity within the rat basolateral amygdala complex and colocalization with the neuropeptide Y Y1 receptor. J Chem Neuroanat 45:50-6
Giesbrecht, Chantelle J; Mackay, James P; Silveira, Heika B et al. (2010) Countervailing modulation of Ih by neuropeptide Y and corticotrophin-releasing factor in basolateral amygdala as a possible mechanism for their effects on stress-related behaviors. J Neurosci 30:16970-82
Rostkowski, Amanda B; Teppen, Tara L; Peterson, Daniel A et al. (2009) Cell-specific expression of neuropeptide Y Y1 receptor immunoreactivity in the rat basolateral amygdala. J Comp Neurol 517:166-76
Sajdyk, Tammy J; Johnson, Philip L; Leitermann, Randy J et al. (2008) Neuropeptide Y in the amygdala induces long-term resilience to stress-induced reductions in social responses but not hypothalamic-adrenal-pituitary axis activity or hyperthermia. J Neurosci 28:893-903
Dimitrov, Eugene L; DeJoseph, M Regina; Brownfield, Mark S et al. (2007) Involvement of neuropeptide Y Y1 receptors in the regulation of neuroendocrine corticotropin-releasing hormone neuronal activity. Endocrinology 148:3666-73