In the brains of mammals, birds and invertebrates, the sensory world is organized into regular neuronal arrays or maps. Common examples are the map of body surface in somatosensory cortex (the so called """"""""homunculus"""""""") and the representation of oriented bars or edges in visual cortex. In these maps, neighboring cells respond to similar features of stimuli in the sensory periphery, and there is usually an orderly progression of the optimal stimulus across the array of neurons. Through the efforts of Hubel, Wiesel and many of their colleagues we now have a fairly comprehensive description of maps of stimulus preference in many areas of cortex, but fundamental questions regarding their development of function remain unanswered despite more that forty years of intense research. Sensory maps in the cortex are established by the orderly arrangement of afferents from the thalamus to layer IV in the cortex. The precise development of these maps requires, at least in part, afferent neuronal activity, and is subject to use-dependent modification that relies on the sensory experience of the animal. The objectives of the research outlined in this proposal are: (1) determine the cellular and synaptic features of the development of thalamocortical connections in mice with normal sensory experience; (2) determine the influence of sensory experience and neuronal activity on the development of thalamocortical synapses; and (3) establish the cellular and synaptic mechanisms for sensory map plasticity at thalamocortical synapses in developing animals. Experiments addressing these questions will be performed in vitro with whole cell patch-clamp techniques from mouse somatosensory cortex using a thalamocortical brain slice preparation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Project (R01)
Project #
5R01MH062639-04
Application #
6708873
Study Section
Special Emphasis Panel (ZRG1-MDCN-7 (01))
Program Officer
Sieber, Beth-Anne
Project Start
2001-02-15
Project End
2006-01-31
Budget Start
2004-02-01
Budget End
2005-01-31
Support Year
4
Fiscal Year
2004
Total Cost
$263,375
Indirect Cost
Name
Baylor College of Medicine
Department
Neurosciences
Type
Schools of Medicine
DUNS #
051113330
City
Houston
State
TX
Country
United States
Zip Code
77030
Fertuzinhos, Sofia; Li, Mingfeng; Kawasawa, Yuka Imamura et al. (2014) Laminar and temporal expression dynamics of coding and noncoding RNAs in the mouse neocortex. Cell Rep 6:938-50
Li, Hong; Fertuzinhos, Sofia; Mohns, Ethan et al. (2013) Laminar and columnar development of barrel cortex relies on thalamocortical neurotransmission. Neuron 79:970-86
Dhande, Onkar S; Bhatt, Shivani; Anishchenko, Anastacia et al. (2012) Role of adenylate cyclase 1 in retinofugal map development. J Comp Neurol 520:1562-83
Dhande, Onkar S; Hua, Ethan W; Guh, Emily et al. (2011) Development of single retinofugal axon arbors in normal and ?2 knock-out mice. J Neurosci 31:3384-99
Li, Hong; Crair, Michael C (2011) How do barrels form in somatosensory cortex? Ann N Y Acad Sci 1225:119-29
Dhande, Onkar S; Crair, Michael C (2011) Transfection of mouse retinal ganglion cells by in vivo electroporation. J Vis Exp :
Chandrasekaran, Anand R; Furuta, Yas; Crair, Michael C (2009) Consequences of axon guidance defects on the development of retinotopic receptive fields in the mouse colliculus. J Physiol 587:953-63
Kurotani, Tohru; Yamada, Kazumasa; Yoshimura, Yumiko et al. (2008) State-dependent bidirectional modification of somatic inhibition in neocortical pyramidal cells. Neuron 57:905-16
Iwasato, Takuji; Inan, Melis; Kanki, Hiroaki et al. (2008) Cortical adenylyl cyclase 1 is required for thalamocortical synapse maturation and aspects of layer IV barrel development. J Neurosci 28:5931-43
Shah, Ruchir D; Crair, Michael C (2008) Retinocollicular synapse maturation and plasticity are regulated by correlated retinal waves. J Neurosci 28:292-303

Showing the most recent 10 out of 26 publications