Synaptic plasticity is a fundamental feature of the nervous system that underlies neural development, adaptation and learning. There is growing evidence that deficits in the mechanisms of synaptic plasticity are involved in the pathophysiology of many psychiatric disorders, from schizophrenia to mood disorders. For this reason, NIMH has established as one of his strategic research priorities the study of brain plasticity at the cellular, synaptic, circuit, and behavioral level, with the final goal of determining the neurobiological bases of these processes. This proposal will study humans and three animal models (flies, mice, rats) to test the novel and provocative idea that synaptic plasticity is adaptive up to a point, but beyond that point, or in vulnerable individuals, it can become maladaptive. The cost of synaptic plasticity is not often considered but may be crucial in the pathophysiology of psychiatric disorders, and will be assessed at the ultrastructural, cellular, circuit, and behavioral level. Our previous NIMH-funded work has established that the overall result of wake plasticity is a net increase in synaptic strength, which is renormalized by sleep. But what happens when plasticity is excessive, for instance because it is extended beyond the physiological range without intervening sleep? Based on preliminary results obtained in both animals and humans, we hypothesize that extended plasticity can lead to negative consequences on neuronal activity (OFF periods, performance deficits) and on cellular function/integrity (cellular stress, ultrastructural abnormalities).
Aim 1 will use rats to test whether plasticity-dependent synaptic overload leads to the occurrence of neuronal OFF periods, local EEG slowing during wake, and performance impairment. It will also establish to what extent these effects are a region- specific consequence of plasticity, rather than a general effect of prolonged wake.
Aim 2 will use high density (hd) EEG in humans to ask whether the local increase in EEG theta waves, which occurs during wake as a result of extended plasticity in specific brain circuits, leads to local performance deficits, locally increased sleep need, and o sleep-dependent restoration of function.
Aim 3 will use flies and mice to test whether extending plasticity by prolonging wakefulness leads to cellular stress and subcellular damage, and whether doing so chronically under sleep restriction conditions leads to lasting cellular damage and cognitive deficits. Plasticity plays a central role in the life of every organism, but its coston neural structure and function may be substantial especially at vulnerable developmental times, such as adolescence, or in vulnerable populations, such as psychiatric patients. Demonstrating the cost of plasticity at the cellular and systems level will have clear practical implications forthe prevention and treatment of mental disorders.
This proposal will study humans and three animal models (flies, mice, rats) to understand at the ultrastructural, cellular, circuit, and behavioral level te 'cost' of synaptic plasticity, an aspect of plasticity that is not often considered but may be crucal in the pathophysiology of psychiatric disorders.
| Bellesi, Michele; de Vivo, Luisa; Koebe, Samuel et al. (2018) Sleep and Wake Affect Glycogen Content and Turnover at Perisynaptic Astrocytic Processes. Front Cell Neurosci 12:308 |
| Bourdon, Allen K; Spano, Giovanna Maria; Marshall, William et al. (2018) Metabolomic analysis of mouse prefrontal cortex reveals upregulated analytes during wakefulness compared to sleep. Sci Rep 8:11225 |
| Honjoh, Sakiko; Sasai, Shuntaro; Schiereck, Shannon S et al. (2018) Regulation of cortical activity and arousal by the matrix cells of the ventromedial thalamic nucleus. Nat Commun 9:2100 |
| Bernardi, Giulio; Siclari, Francesca; Handjaras, Giacomo et al. (2018) Local and Widespread Slow Waves in Stable NREM Sleep: Evidence for Distinct Regulation Mechanisms. Front Hum Neurosci 12:248 |
| Funk, Chadd M; Peelman, Kayla; Bellesi, Michele et al. (2017) Role of Somatostatin-Positive Cortical Interneurons in the Generation of Sleep Slow Waves. J Neurosci 37:9132-9148 |
| Siclari, Francesca; Tononi, Giulio (2017) Local aspects of sleep and wakefulness. Curr Opin Neurobiol 44:222-227 |
| de Vivo, Luisa; Bellesi, Michele; Marshall, William et al. (2017) Ultrastructural evidence for synaptic scaling across the wake/sleep cycle. Science 355:507-510 |
| Honjoh, Sakiko; de Vivo, Luisa; Okuno, Hiroyuki et al. (2017) Higher Arc Nucleus-to-Cytoplasm Ratio during Sleep in the Superficial Layers of the Mouse Cortex. Front Neural Circuits 11:60 |
| Nagai, Hirotaka; de Vivo, Luisa; Bellesi, Michele et al. (2017) Sleep Consolidates Motor Learning of Complex Movement Sequences in Mice. Sleep 40: |
| Nir, Yuval; Andrillon, Thomas; Marmelshtein, Amit et al. (2017) Selective neuronal lapses precede human cognitive lapses following sleep deprivation. Nat Med 23:1474-1480 |
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