Neuropsychiatric disorders are the leading cause of disease burden in the United States and Canada, far outpacing other maladies such as cardiovascular disease and cancer (WHO statistics). Progress in treating neuropsychiatric disorders is severely hampered by our lack of basic knowledge related to their underlying causes. Defects in dendritic spine morphogenesis, a process regulated by dynamic actin remodeling, is a common feature of these disorders and is also associated with stress, which may precipitate disorders such as schizophrenia. Moreover, it is increasingly clear that disruptions in genes that regulate signaling to excitatory synaptic actin are risk factors for schizophrenia, autism, and intellectual disability. Arp2/3 complex is enriched in dendritic spines and stimulates the formation of branched actin downstream of many genes implicated neuropsychiatric disorders. Recently we published that the conditional loss of Arp2/3 in mice leads to the progressive development of multiple synaptic and behavioral phenotypes relevant to models of schizophrenia. Many of the schizophrenia-related behaviors are normalized by the antipsychotics clozapine and haloperidol.
The specific aims of this grant build on these exciting findings to address fundamental questions of how SZ-related phenotypes evolve at the synaptic and circuit level and how this is influenced by chronic stress. We anticipate the results of these aims will bridge our knowledge gap regarding how SZ-like phenotypes emerge in vivo, leading to new future directions for the prevention and possible treatments of the disorder.

Public Health Relevance

Genetic evidence suggests that an accumulation of genetic interactions between multiple allelic variants can predispose individuals to schizophrenia (SZ), implying that SZ is a signaling network or pathway disorder. The realization that disorders such as SZ are genetically heterogeneous presents a formidable problem. However, there is hope that identifying mutations associated with SZ may lead to the identification of pathways that are commonly disrupted in the illness. Unfortunately, pathway disorders are difficult to model by mutation of single regulatory nodes due to the highly connected and compensatory nature of most biological networks. Thus an innovative and informative approach is to conditionally target the final downstream output of multiple pathways implicated in SZ. This approach can clarify which pathways are likely to contribute to individual features of the disorder. Recently we demonstrated that abnormal signaling to Arp2/3 is one such pathway, and that the disruption of Arp2/3 can recapitulate the functional manifestations of many upstream candidate genes previously associated with SZ. We are now in a position to answer fundamental questions of how dysregulated Arp2/3 activity leads to synaptic and behavioral SZ-like phenotypes. Understanding of the neurobiology underlying the onset and progression of SZ is highly relevant to the mission of NIH.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Project (R01)
Project #
1R01MH103374-01
Application #
8672933
Study Section
Special Emphasis Panel (ZRG1-MDCN-P (57))
Program Officer
Asanuma, Chiiko
Project Start
2014-02-10
Project End
2019-01-31
Budget Start
2014-02-10
Budget End
2015-01-31
Support Year
1
Fiscal Year
2014
Total Cost
$392,500
Indirect Cost
$142,500
Name
Duke University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
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Yan, Zhen; Kim, Eunjoon; Datta, Dibyadeep et al. (2016) Synaptic Actin Dysregulation, a Convergent Mechanism of Mental Disorders? J Neurosci 36:11411-11417
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