The long term objective of this project is to prevent or attempt to reduce the incidence of cholera where people must depend on natural ground water especially under the abnormal circumstances such as after flooding or post natural disaster situations. A simple filtration method developed and optimized in the laboratory will be evaluated in the field to demonstrate the effciacy in reducing the number of V. cholerae cells in household water in Matlab, Bangladesh. Sari cloth, worn by most women in the villages in Bangladesh was used as a filter and will be to used to remove V. cholerae associated plankton and particles present in surface water. This device is expected to be the most convenient for the villagers without economic, social and cultural impediments.

Agency
National Institute of Health (NIH)
Institute
National Institute of Nursing Research (NINR)
Type
Research Project (R01)
Project #
5R01NR004527-03
Application #
6186870
Study Section
Special Emphasis Panel (ZRG4-EDC-2 (04))
Program Officer
Hare, Martha L
Project Start
1998-09-15
Project End
2003-07-31
Budget Start
2000-08-01
Budget End
2003-07-31
Support Year
3
Fiscal Year
2000
Total Cost
$249,366
Indirect Cost
Name
University of MD Biotechnology Institute
Department
Type
Organized Research Units
DUNS #
City
Baltimore
State
MD
Country
United States
Zip Code
21202
Gil, Ana I; Louis, Valerie R; Rivera, Irma N G et al. (2004) Occurrence and distribution of Vibrio cholerae in the coastal environment of Peru. Environ Microbiol 6:699-706
Talledo, Miguel; Rivera, Irma N G; Lipp, Erin K et al. (2003) Characterization of a Vibrio cholerae phage isolated from the coastal water of Peru. Environ Microbiol 5:350-4
Lipp, Erin K; Rivera, Irma N G; Gil, Ana I et al. (2003) Direct detection of Vibrio cholerae and ctxA in Peruvian coastal water and plankton by PCR. Appl Environ Microbiol 69:3676-80
Heidelberg, J F; Heidelberg, K B; Colwell, R R (2002) Seasonality of Chesapeake Bay bacterioplankton species. Appl Environ Microbiol 68:5488-97
Heidelberg, J F; Heidelberg, K B; Colwell, R R (2002) Bacteria of the gamma-subclass Proteobacteria associated with zooplankton in Chesapeake Bay. Appl Environ Microbiol 68:5498-507
Chaiyanan, S; Chaiyanan, S; Huq, A et al. (2001) Viability of the nonculturable Vibrio cholerae O1 and O139. Syst Appl Microbiol 24:331-41