We plan to exploit newly developed possibilities to learn about the machinery of synaptic transmission by use of the neuromuscularjunction on the ceratomandibularis muscle of the lizard. We plan to use focal extracellular recordings and patch clamps at the terminal boutons of the nerve ending to: 1) determine the type (or types) of calcium channels and their location 2) determine the relationship between calcium current and transmitter release 3) determine the relationship between the sub-membrane Ca concentration and transmitter release 4) identify a putative Na-Ca exchange current 5) look for evidence of vesicular fusion by video contrast enhancement and image processing 6) test for insertion of new channels by fusion of vesicles into the surface membrane of the bouton. We will simulate our experiments with a powerful new computer program in order o gain insight into our interpretations of the currents, Ca concentration profiles, and vesicle fusion machinery.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS003437-28
Application #
3393287
Study Section
Physiology Study Section (PHY)
Project Start
1978-09-01
Project End
1992-08-31
Budget Start
1988-09-25
Budget End
1989-08-31
Support Year
28
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Duke University
Department
Type
Schools of Medicine
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705
Lindgren, C A; Moore, J W (1989) Identification of ionic currents at presynaptic nerve endings of the lizard. J Physiol 414:201-22
Hayashi, J H; Moore, J W; Stuart, A E (1985) Adaptation in the input-output relation of the synapse made by the barnacle's photoreceptor. J Physiol 368:179-95