Abstract

One of the goals of this project is to understand the mechanisms by which contraction is initiated and controlled by potential changes of the membranous system of skeletal muscle. Electrical and membranous properties of the transverse tubular system (T-system) and sarcoplasmic reticulum will be explored by using optical methods (fluorescence dye absorption and external fluorescence method) as well as by the conventional electrophysiological techniques. We would particularly like to know details about the radial conduction of action potential through the T-system and about the membrane potential changes of sarcoplasmic reticulum during excitation-contraction coupling. Another goal of this project is to investigate the mechanism of fatigue in synaptic transmission by using the squid stellate ganglion.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS008601-16
Application #
3393785
Study Section
Physiology Study Section (PHY)
Project Start
1978-12-01
Project End
1986-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
16
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Purdue University
Department
Type
Schools of Arts and Sciences
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907

  Publications

Enz, A; Goldstein, M; Meller, E (1990) Dopamine agonist-induced elevation of striatal acetylcholine: relationship between receptor occupancy and response in normal and denervated rat striatum. Mol Pharmacol 37:560-5
Meller, E; Goldstein, M; Bohmaker, K (1990) Receptor reserve for 5-hydroxytryptamine1A-mediated inhibition of serotonin synthesis: possible relationship to anxiolytic properties of 5-hydroxytryptamine1A agonists. Mol Pharmacol 37:231-7
Carlson, C G; Leonard, R J (1989) Developmental changes in the open time and conductance of acetylcholine receptors in aneural cultured Xenopus myocytes treated with cycloheximide or tunicamycin. Brain Res Dev Brain Res 46:61-8
Leonard, R J; Nakajima, S; Nakajima, Y et al. (1988) Early development of two types of nicotinic acetylcholine receptors. J Neurosci 8:4038-48
Meller, E; Goldstein, M; Friedhoff, A J et al. (1988) N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ): a new tool to probe CNS receptor function. Adv Exp Med Biol 235:121-36
Meller, E; Enz, A; Goldstein, M (1988) Absence of receptor reserve at striatal dopamine receptors regulating cholinergic neuronal activity. Eur J Pharmacol 155:151-4
Takahashi, T; Nakajima, Y; Hirosawa, K et al. (1987) Structure and physiology of developing neuromuscular synapses in culture. J Neurosci 7:473-81
Nakajima, Y; Nakajima, S; Leonard, R J et al. (1986) Acetylcholine raises excitability by inhibiting the fast transient potassium current in cultured hippocampal neurons. Proc Natl Acad Sci U S A 83:3022-6
Greenberg, A S; Nakajima, S; Nakajima, Y (1985) Functional properties of newly inserted acetylcholine receptors in embryonic Xenopus muscle cells. Brain Res 351:289-96