Abstract

The purpose of this study will be to isolate and characterize the major basic proteins of peripheral nerve myelin and determine their immunogenic properties especially with regard to their ability to produce experimental allergic neuritis in Lewis rats. By isolating immunogenic peptide fragments, we will localize the antigenic and neuritogenic determinants and investigate the properties of these determinants. Using the P2 protein or peptides we will attempt to manipulate the immune response so as to affect and particularly to treat the disorder. We will study the circulating antibody response and cell-mediated immune response to the neuritogenic determinants during the induction and treatment of experimental allergic neuritis. Since experimental allergic neuritis is the appropriate model for human idiopathic polyneuritis, we hope to gain insight into the nature of the human disease so as to design more effective treatment for this condition.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS011867-12
Application #
3394640
Study Section
Neurology B Subcommittee 1 (NEUB)
Project Start
1977-05-01
Project End
1986-07-31
Budget Start
1985-05-01
Budget End
1986-07-31
Support Year
12
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Medical University of South Carolina
Department
Type
Schools of Medicine
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425

  Publications

Powell, H C; Myers, R R; Mizisin, A P et al. (1991) Response of the axon and barrier endothelium to experimental allergic neuritis induced by autoreactive T cell lines. Acta Neuropathol 82:364-77
Powell, H C; Olee, T; Brostoff, S W et al. (1991) Comparative histology of experimental allergic neuritis induced with minimum length neuritogenic peptides by adoptive transfer with sensitized cells or direct sensitization. J Neuropathol Exp Neurol 50:658-74
Olee, T; Powell, H C; Brostoff, S W (1990) New minimum length requirement for a T cell epitope for experimental allergic neuritis. J Neuroimmunol 27:187-90
Ellerman, K; Brostoff, S (1988) A suppressor cell line that prevents the adoptive transfer of EAE in Lewis rats. Ann N Y Acad Sci 540:364-6
Brostoff, S W; White, T M; Powers, J M (1988) Treatment of clinical experimental allergic encephalomyelitis in the rat using fragments and combinations of monoclonal antibodies. J Neuroimmunol 17:167-73
Olee, T; Powers, J M; Brostoff, S W (1988) A T cell epitope for experimental allergic neuritis. J Neuroimmunol 19:167-73
Weise, M J; Carnegie, P R (1988) An approach to searching protein sequences for superfamily relationships or chance similarities relevant to the molecular mimicry hypothesis: application to the basic proteins of myelin. J Neurochem 51:1267-73
Sedgwick, J; Brostoff, S; Mason, D (1987) Experimental allergic encephalomyelitis in the absence of a classical delayed-type hypersensitivity reaction. Severe paralytic disease correlates with the presence of interleukin 2 receptor-positive cells infiltrating the central nervous system. J Exp Med 165:1058-75
Robinson, A P; White, T M; Mason, D W (1986) Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology 57:239-47
Robinson, A P; White, T M; Mason, D W (1986) MRC OX-43: a monoclonal antibody which reacts with all vascular endothelium in the rat except that of brain capillaries. Immunology 57:231-7

Showing the most recent 10 out of 12 publications