Abstract

The objective of this research is to identify chemical markers for specific populations of auditory synopatic terminals in the superior olivary complex, cochlear nucleus and cochlea so that we may map their distribution and location on specific classes of auditory neurons and describe differences in their chemical properties, especially those related to their neurotransmitters. These studies will examine the localization of radiolabeled compounds related to putative neurotransmitter amino acids with light and electron microscopic autoradiography. A fresh brain slice preparation in cats and gerbils and an in vivo cochlear preparation in gerbils will be used. Conditions affecting the accumulation and release of label will be characterized to determine whether the labeled compound may belong to one of the various categories of neurotransmitter related compounds, or to provide evidence of metabolic properties which may be related to transmitters.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
7R01NS014503-11
Application #
3395582
Study Section
Hearing Research Study Section (HAR)
Project Start
1987-08-01
Project End
1988-03-31
Budget Start
1987-08-01
Budget End
1988-03-31
Support Year
11
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Kawahara, H; Cadrin, M; Perry, G et al. (1990) Role of cytokeratin intermediate filaments in transhepatic transport and canalicular secretion. Hepatology 11:435-48
Yu, S M; Schwartz, I R (1989) An improved flat embedding technique for immunoelectron microscopy. Stain Technol 64:143-6
Morandi, A; Los, B; Osofsky, L et al. (1989) Ubiquitin and heat shock proteins in cultured nervous tissue after different stress conditions. Prog Clin Biol Res 317:819-27
Helfert, R H; Schwartz, I R; Ryan, A F (1988) Ultrastructural characterization of gerbil olivocochlear neurons based on differential uptake of 3H-D-aspartic acid and a wheatgerm agglutinin-horseradish peroxidase conjugate from the cochlea. J Neurosci 8:3111-23
Manetto, V; Sternberger, N H; Perry, G et al. (1988) Phosphorylation of neurofilaments is altered in amyotrophic lateral sclerosis. J Neuropathol Exp Neurol 47:642-53
Ryan, A F; Schwartz, I R; Helfert, R H et al. (1987) Selective retrograde labeling of lateral olivocochlear neurons in the brainstem based on preferential uptake of 3H-D-aspartic acid in the cochlea. J Comp Neurol 255:606-16
Helfert, R H; Schwartz, I R (1987) Morphological features of five neuronal classes in the gerbil lateral superior olive. Am J Anat 179:55-69
Helfert, R H; Schwartz, I R (1986) Morphological evidence for the existence of multiple neuronal classes in the cat lateral superior olivary nucleus. J Comp Neurol 244:533-49
Ryan, A F; Schwartz, I R (1986) Nipecotic acid: preferential accumulation in the cochlea by GABA uptake systems and selective retrograde transport to brainstem. Brain Res 399:399-403
Schwartz, I R; Ryan, A F (1986) Amino acid labeling patterns in the efferent innervation of the cochlea: an electron microscopic autoradiographic study. J Comp Neurol 246:500-12