Abstract

By means of standard electrophysiological methods, including constant-current and voltage-clamp techniques, the actions of chiriquitoxin and other analogues of tetrodotoxin will be studied on a variety of excitable membranes including the frog skeletal muscle fiber and several well-studied axons. The objective is the further understanding of the mechanism of interaction of tetrodotoxin and related compounds with the reciprocal membrane receptor, and to estimate the density and spatial relations of some of the major ionic conductance channels in the membranes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS014551-06
Application #
3395625
Study Section
Physiology Study Section (PHY)
Project Start
1978-07-01
Project End
1987-06-30
Budget Start
1985-01-01
Budget End
1987-06-30
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Suny Downstate Medical Center
Department
Type
Schools of Medicine
DUNS #
068552207
City
Brooklyn
State
NY
Country
United States
Zip Code
11203

  Publications

Wu, B Q; Yang, L; Kao, C Y et al. (1996) 11-Oxo-tetrodotoxin and a specifically labelled 3H-tetrodotoxin. Toxicon 34:407-16
Yang, L; Kao, C Y; Oshima, Y (1992) Actions of decarbamoyloxysaxitoxin and decarbamoylneosaxitoxin on the frog skeletal muscle fiber. Toxicon 30:645-52
Yang, L; Kao, C Y; Yasumoto, T (1992) Actions of 6-epitetrodotoxin and 11-deoxytetrodotoxin on the frog skeletal muscle fiber. Toxicon 30:635-43
Yang, L; Kao, C Y (1992) Actions of chiriquitoxin on frog skeletal muscle fibers and implications for the tetrodotoxin/saxitoxin receptor. J Gen Physiol 100:609-22
Hu, S L; Kao, C Y (1991) Interactions of neosaxitoxin with the sodium channel of the frog skeletal muscle fiber. J Gen Physiol 97:561-78
Kao, C Y; Salwen, M J; Hu, S L et al. (1989) Diamphidia toxin, the bushmen's arrow poison: possible mechanism of prey-killing. Toxicon 27:1351-66
Hu, S L; Kao, C Y; Koehn, F E et al. (1987) Inaction of saxitoxin-oximes on the sodium channel of frog skeletal muscle fibers. Toxicon 25:159-65
Hu, S L; Kao, C Y (1986) The pH dependence of the tetrodotoxin-blockade of the sodium channel and implications for toxin binding. Toxicon 24:25-31
Kao, C Y (1986) Structure-activity relations of tetrodotoxin, saxitoxin, and analogues. Ann N Y Acad Sci 479:52-67
Kao, C Y; Kao, P N; James-Kracke, M R et al. (1985) Actions of epimers of 12-(OH)-reduced saxitoxin and of 11-(OSO3)-saxitoxin on squid axon. Toxicon 23:647-55

Showing the most recent 10 out of 12 publications