The objectives are to define the ultrastructural cytologic distribution of (Na+ + K+)ATPase in the mouse nervous system and developmental changes in this distribution in cerebellum, optic nerve, and in tissue culture. This will lead to understanding cell membrane differentiation related to cation transport function in the brain. Mouse brain (Na+ + K+)-ATPase will be purified by detergent extraction and isopycnic sedimentation. The catalytic polypeptide will be identified by Na+-dependent phosphorylation and separated by polyacrylamide gel elebrophoresis. Specific antisera to the holoenzyme and catalytic unit will be raised in rabbits. Immunocytochemical studies of mouse nervous system will be performed with tissue specific antibodies at the light and electron microscopy levels by means of peroxidase-antiperoxidase and protein A-gold methods. Antibodies will be purified by immunoafinity chromatography with purified catalysic polypeptide. This project involves disciplines of brain anatomy, ontogeny, biochemistry, immunology, and neurocellular biology.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS015935-07
Application #
3396583
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1979-12-01
Project End
1988-06-30
Budget Start
1987-01-01
Budget End
1988-06-30
Support Year
7
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
Schools of Medicine
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109