Abstract

We will terminate the amino acid sequence and the glycosylation sites of the human N-terminal fragment of proopiocortin. If some material is left (we have access to only 1,000 human pituitaries per year), we will provide it to other investigators to study its effects on adrenald. We will complete the purification of porcine N-terminal fragment and we will carry out its complete amino acid sequence plus its sites of glycosylation. We will also purify 100-200 mg of this porcine material in order to make it available to as many investigators as possible. We believe we will need at least 20-30 kg of porcine pituitaries to reach this goal.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS016315-06
Application #
3396808
Study Section
Endocrinology Study Section (END)
Project Start
1979-12-01
Project End
1985-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Clinical Research Institute of Montreal
Department
Type
DUNS #
City
Montreal
State
QC
Country
Canada
Zip Code
H2 1R7

  Publications

Gaspar, L; Chan, J S; Seidah, N G et al. (1989) Peptides related to the N-terminus of pro-opiomelanocortin in the human adrenal medulla. Clin Invest Med 12:90-8
Cromlish, J A; Seidah, N G; Marcinkiewicz, M et al. (1987) Human pituitary tryptase: molecular forms, NH2-terminal sequence, immunocytochemical localization, and specificity with prohormone and fluorogenic substrates. J Biol Chem 262:1363-73
Seidah, N G; Donohue-Rolfe, A; Lazure, C et al. (1986) Complete amino acid sequence of Shigella toxin B-chain. A novel polypeptide containing 69 amino acids and one disulfide bridge. J Biol Chem 261:13928-31
Bennett, H P; Seidah, N G; Benjannet, S et al. (1986) Reinvestigation of the disulfide bridge arrangement in human pro-opiomelanocortin N-terminal segment (hNT 1-76). Int J Pept Protein Res 27:306-13
Cromlish, J A; Seidah, N G; Chretien, M (1986) A novel serine protease (IRCM-serine protease 1) from porcine neurointermediate and anterior pituitary lobes. Isolation, polypeptide chain structure, inhibitor sensitivity, and substrate specificity with fluorogenic peptide substrates. J Biol Chem 261:10850-8
Burbach, J P; Seidah, N G; Chretien, M (1986) Isolation and primary structure of novel neurointermediate pituitary peptides derived from the C-terminal of the rat vasopressin-neurophysin precursor (propressophysin). Eur J Biochem 156:137-42
Cromlish, J A; Seidah, N G; Chretien, M (1986) Selective cleavage of human ACTH, beta-lipotropin, and the N-terminal glycopeptide at pairs of basic residues by IRCM-serine protease 1. Subcellular localization in small and large vesicles. J Biol Chem 261:10859-70
Gaspar, L; Chan, J S; Seidah, N G et al. (1985) Characterization of peptides derived from pro-opiomelanocortin in the biological fluids of a patient with Nelson's syndrome. Clin Invest Med 8:167-75
Marcinkiewicz, M; Benjannet, S; Seidah, N G et al. (1985) Immunocytochemical localization of a novel pituitary protein (7B2) within the rat brain and hypophysis. J Histochem Cytochem 33:1219-26
Marcinkiewicz, M; Benjannet, S; Sikstrom, R A et al. (1985) Immunoreactivity of vasopressin and a novel pituitary protein '7B2' in Long-Evans and Brattleboro rat hypothalamus and hypophysis. Neurosci Lett 60:7-12