Many lines of evidence point to the cerebellar cortex as having a parasagittal organization. This evidence includes anatomical tracing studies, electrophysiological single unit studies, histochemical and immunohistochemical analyses of the cerebellum. Even though these various methodologies have been successful in demonstrating parasagittal organization of one form or another, there have been relatively few attempts to compare, collate, and synthesize the data from these different methodologies. It is absolutely essential to attack this problem if we are ever to determine the true nature of cerebellar parasagittal organization and its functional significance. We have been using anatomical tracing techniques to study the parasagittal organization of the cerebellum in the rat and mouse. By using these data as a starting point we propose to compare and contrast, in a detailed and thorough way the parasagittal organizational schemes which result when different methodological approaches are applied to the same species. This represents a logical and important extension of the work we have been doing on afferent organization to the cerebellum. Toward this end we plan to carry out the following experiments. (1) Compare and analyze, in the same animal, the immunohistochemical staining pattern of Purkinje cells obtained with the monoclonal antibody Q113 and Q155, to the pattern of climbing fiber afferents to the cerebellum as demonstrated with WGA-HRP anterograde tracing techniques. (2) Carry out a detailed analysis of one lobule of the cerebellum (lobule VIII and the copula pyramidis) with regard to the pattern of its climbing fiber and mossy fiber inputs as studied with WGA-HRP retrograde tracing techniques and the immunohistochemical staining pattern of this lobule with mab Q113. (3) Conduct a similar comparative analysis with 5' nucleotidase histochemical staining patterns and the pattern of afferent input. (4) Determine the dependency, if any, of these staining patterns (5' nucleotidase, mab Q113, and/or Q155) on the integrity of the olivocerebellar projection by making unilateral chemical lesions of the inferior olivary complex.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS016531-04
Application #
3396946
Study Section
Neurology A Study Section (NEUA)
Project Start
1983-12-01
Project End
1990-08-31
Budget Start
1986-12-01
Budget End
1988-08-31
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Thomas Jefferson University
Department
Type
Schools of Medicine
DUNS #
061197161
City
Philadelphia
State
PA
Country
United States
Zip Code
19107
Hawkes, R; Eisenman, L M (1997) Stripes and zones: the origins of regionalization of the adult cerebellum. Perspect Dev Neurobiol 5:95-105
Paradies, M A; Grishkat, H; Smeyne, R J et al. (1996) Correspondence between L7-lacZ-expressing Purkinje cells and labeled olivocerebellar fibers during late embryogenesis in the mouse. J Comp Neurol 374:451-66
Akintunde, A; Eisenman, L M (1994) External cuneocerebellar projection and Purkinje cell zebrin II bands: a direct comparison of parasagittal banding in the mouse cerebellum. J Chem Neuroanat 7:75-86
Eisenman, L M; Hawkes, R (1993) Antigenic compartmentation in the mouse cerebellar cortex: zebrin and HNK-1 reveal a complex, overlapping molecular topography. J Comp Neurol 335:586-605
Eisenman, L M; Schalekamp, M P; Voogd, J (1991) Development of the cerebellar cortical efferent projection: an in-vitro anterograde tracing study in rat brain slices. Brain Res Dev Brain Res 60:261-6
Eisenman, L M; Hawkes, R (1989) 5'-Nucleotidase and the mabQ113 antigen share a common distribution in the cerebellar cortex of the mouse. Neuroscience 31:231-5
Eisenman, L M (1988) Histochemical localization of 5'-nucleotidase in the reeler mutant mouse. Neurosci Lett 94:70-5
Heckroth, J A; Eisenman, L M (1988) The olivocerebellar projection in 'lurcher' mutant mice. Neurosci Lett 85:199-204
Heckroth, J A; Eisenman, L M (1988) Parasagittal organization of mossy fiber collaterals in the cerebellum of the mouse. J Comp Neurol 270:385-94
Blatt, G J; Eisenman, L M (1988) Topographic and zonal organization of the olivocerebellar projection in the reeler mutant mouse. J Comp Neurol 267:603-15

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