The first set of proposed experiments is designed to compare and contrast synaptogenesis occurring in a regenerating system, the goldfish retinotectal system, with lesion induced reorganization occurring by collateral sprouting in a non-regenerating system, the rat interpeduncular nucleus (IPN). The specific issues are related to a comparison of the factors regulating reestablishment of specific numbers and patterns of connections by regenerating goldfish retinal axons with the factors regulating collateral sprouting and reactive reinnervation by intact neurons after lesions in mammalian CNS. This information is essential in order to predict the functional consequences of lesion induced synaptogenesis in the adult CNS. A second goal is to determine those cellular processes which distinguish the response of the rat retinal ganglion cell, axotomized and induced to elongate its axon via implantation techniques, from the axotomized but non-regenerating rat retinal ganglion cell and from the regenerating goldfish retinal ganglion cell. These studies should contribute to an elucidation of the processes which account for the robust and directed growth of the axotomized goldfish optic axons and those which account for the normal failure of this growth in the axotomized mammalian central axons.

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National Institute of Neurological Disorders and Stroke (NINDS)
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Neurology B Subcommittee 1 (NEUB)
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Allegheny University of Health Sciences
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Murphy, C A; DiCamillo, A M; Haun, F et al. (1996) Lesion of the habenular efferent pathway produces anxiety and locomotor hyperactivity in rats: a comparison of the effects of neonatal and adult lesions. Behav Brain Res 81:43-52
Battisti, W P; Wang, J; Bozek, K et al. (1995) Macrophages, microglia, and astrocytes are rapidly activated after crush injury of the goldfish optic nerve: a light and electron microscopic analysis. J Comp Neurol 354:306-20
Wang, J; Murray, M; Grafstein, B (1995) Cranial meninges of goldfish: age-related changes in morphology of meningeal cells and accumulation of surfactant-like multilamellar bodies. Cell Tissue Res 281:349-58
Thornton, E W; Murray, M; Connors-Eckenrode, T et al. (1994) Dissociation of behavioral changes in rats resulting from lesions of the habenula versus fasciculus retroflexus and their possible anatomical substrates. Behav Neurosci 108:1150-62
Murray, M; Bozek, K; Haun, F (1994) Transient and permanent patterns of expression of the low-affinity neurotrophin receptor in the interpeduncular nucleus of the rat. Exp Neurol 127:184-90
Haun, F; Eckenrode, T C; Murray, M (1992) Habenula and thalamus cell transplants restore normal sleep behaviors disrupted by denervation of the interpeduncular nucleus. J Neurosci 12:3282-90
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Eckenrode, T C; Murray, M; Haun, F (1992) Habenula and thalamus cell transplants mediate different specific patterns of innervation in the interpeduncular nucleus. J Neurosci 12:3272-81
Lavie, V; Murray, M; Solomon, A et al. (1990) Growth of injured rabbit optic axons within their degenerating optic nerve. J Comp Neurol 298:293-314
Henken, D B; Battisti, W P; Chesselet, M F et al. (1990) Expression of beta-preprotachykinin mRNA and tachykinins in rat dorsal root ganglion cells following peripheral or central axotomy. Neuroscience 39:733-42

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