Abstract

A comprehensive analysis of hippocampal interneurons is proposed in this application. Intracellular recording and staining methods will be used to identify and characterize various interneuron populations in the CA2-CA3a region of rat hippocampal slices (in vitro). The efects that these local circuit neurons exert on pyramidal cells will be studied during simultaneous intracellular penetrations of interneuron-pyramidal cell pairs. Interneuron influences on phyramidal cells will be compared to those of stimulus-induced synaptic inputs and pressure-ejected putative neurotransmitters. In other experiments, effects of various drugs (enkephalin, acetylcholine, kainate) and environmental treatments (hypoxia, repetitive stimulation) on interneurons will be investigated. Applying these morphological, electrophysiological, and pharmacological techniques, we can begin to study CNS neurons within the context of their functional circuits and local environments. Thus, it may be possible to gain a better understanding of the richness and complexity of CNS function.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS018895-05
Application #
3398938
Study Section
Neurology B Subcommittee 1 (NEUB)
Project Start
1983-01-01
Project End
1988-12-31
Budget Start
1987-01-01
Budget End
1987-12-31
Support Year
5
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Kinoshita, Yoshito; Wenzel, H Jurgen; Kinoshita, Chizuru et al. (2012) Acute, but reversible, kainic acid-induced DNA damage in hippocampal CA1 pyramidal cells of p53-deficient mice. Epilepsia 53 Suppl 1:125-33
Knight, Leena S; Wenzel, H Jurgen; Schwartzkroin, Philip A (2012) Inhibition and interneuron distribution in the dentate gyrus of p35 knockout mice. Epilepsia 53 Suppl 1:161-70
Wenzel, H Jurgen; Tamse, Catherine T; Schwartzkroin, Philip A (2007) Dentate development in organotypic hippocampal slice cultures from p35 knockout mice. Dev Neurosci 29:99-112
Tschuluun, Naranzogt; Wenzel, H Jurgen; Schwartzkroin, Philip A (2007) Irradiation exacerbates cortical cytopathology in the Eker rat model of tuberous sclerosis complex, but does not induce hyperexcitability. Epilepsy Res 73:53-64
Tschuluun, N; Wenzel, J H; Katleba, K et al. (2005) Initiation and spread of epileptiform discharges in the methylazoxymethanol acetate rat model of cortical dysplasia: functional and structural connectivity between CA1 heterotopia and hippocampus/neocortex. Neuroscience 133:327-42
Wenzel, H Jurgen; Patel, Leena S; Robbins, Carol A et al. (2004) Morphology of cerebral lesions in the Eker rat model of tuberous sclerosis. Acta Neuropathol (Berl) 108:97-108
Patel, Leena S; Wenzel, H Jurgen; Schwartzkroin, Philip A (2004) Physiological and morphological characterization of dentate granule cells in the p35 knock-out mouse hippocampus: evidence for an epileptic circuit. J Neurosci 24:9005-14
Lopantsev, V; Wenzel, H J; Cole, T B et al. (2003) Lack of vesicular zinc in mossy fibers does not affect synaptic excitability of CA3 pyramidal cells in zinc transporter 3 knockout mice. Neuroscience 116:237-48
Lopantsev, Valeri; Tempel, Bruce L; Schwartzkroin, Philip A (2003) Hyperexcitability of CA3 pyramidal cells in mice lacking the potassium channel subunit Kv1.1. Epilepsia 44:1506-12
Szot, P; White, S S; McCarthy, E B et al. (2001) Behavioral and metabolic features of repetitive seizures in immature and mature rats. Epilepsy Res 46:191-203

Showing the most recent 10 out of 73 publications