The program of research proposed in this investigation will employ correlative immunocytochemistry (both light and electron microscopic) and radio-immunoassay to delineate the cortiicotropin releasing factor (CRF) neuronal systems of the central nervous system. CRF immunopositive neurons have been demonstrated to reside in the paraventricular nucleus (PVN) of the hypothalamus and a major fiber projection from these cells was traced to the perivascular contact zone of the median eminence (ME) and proximal neural stem. An analysis of the interrelationships of the CRF system with neurons that produce other peptide hormones (especially vasopressin and ACTH) both within the PVN and the ME is proposed. This program of research will confront such fundamental issues as: 1) What are the interrelationships of the CRF system in the hypothalamus? 2) Do extrahypothalamic CRF neuronal systems exist? If so, how do these neurons interconnect with known systems related to neurogenic stress? 3) Does the hypothalamic CRF system demonstrate a circadian rhythmicity similar to that of ACTH and corticosteroids? Is there a sexual dimorphism of the CRF system? 4) What are the effects of experimental manipulation (e.g. hypophysectomy, adrenalectomy, corticosterone or dexamethasone treatment. ether and restraint stress, ablation of the suprachiasmatic nucleus) on the hypothalamo-pituitary- adrenal systems? This program of research will provide answers to these very basic, but important definitions of the neuroendocrine component of this endocrine axis. Basic information gleaned from these studies will provide the foundation to the understanding of clinical endocrinopathies related to the brain-pituitary-adrenal axis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS019266-03
Application #
3399280
Study Section
Endocrinology Study Section (END)
Project Start
1983-12-01
Project End
1986-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Missouri-Columbia
Department
Type
Schools of Medicine
DUNS #
112205955
City
Columbia
State
MO
Country
United States
Zip Code
65211
Phelix, C F; Liposits, Z; Paull, W K (1994) Catecholamine-CRF synaptic interaction in a septal bed nucleus: afferents of neurons in the bed nucleus of the stria terminalis. Brain Res Bull 33:109-19
Phelix, C F; Liposits, Z; Paull, W K (1992) Serotonin-CRF interaction in the bed nucleus of the stria terminalis: a light microscopic double-label immunocytochemical analysis. Brain Res Bull 28:943-8
Phelix, C F; Liposits, Z; Paull, W K (1992) Monoamine innervation of bed nucleus of stria terminalis: an electron microscopic investigation. Brain Res Bull 28:949-65
Phelix, C F; Paull, W K (1991) Anterograde neuroanatomical tract tracing with central nervous system injections of immunoglobulin G: a light and electron microscopic evaluation. Brain Res Bull 27:255-60
Liposits, Z; Kallo, I; Coen, C W et al. (1990) Ultrastructural analysis of estrogen receptor immunoreactive neurons in the medial preoptic area of the female rat brain. Histochemistry 93:233-9
Liposits, Z (1990) Ultrastructural immunocytochemistry of the hypothalamic corticotropin releasing hormone synthesizing system. Anatomical basis of neuronal and humoral regulatory mechanisms. Prog Histochem Cytochem 21:1-98
Liposits, Z; Kallo, I; Barkovics-Kallo, M et al. (1990) Innervation of somatostatin synthesizing neurons by adrenergic, phenylethanolamine-N-methyltransferase (PNMT)-immunoreactive axons in the anterior periventricular nucleus of the rat hypothalamus. Histochemistry 94:13-20
Phelix, C F; Paull, W K (1990) Demonstration of distinct corticotropin releasing factor--containing neuron populations in the bed nucleus of the stria terminalis. A light and electron microscopic immunocytochemical study in the rat. Histochemistry 94:345-64
Liposits, Z; Paull, W K (1989) Association of dopaminergic fibers with corticotropin releasing hormone (CRH)-synthesizing neurons in the paraventricular nucleus of the rat hypothalamus. Histochemistry 93:119-27
Liposits, Z; Sievers, L; Paull, W K (1988) Neuropeptide-Y and ACTH-immunoreactive innervation of corticotropin releasing factor (CRF)-synthesizing neurons in the hypothalamus of the rat. An immunocytochemical analysis at the light and electron microscopic levels. Histochemistry 88:227-34

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