The present research proposal continues a series of studies on the differentiation of cutaneous sensory innervation during normal development and compares this process with that of regeneration of sensory receptors. Our baseline studies have used monkey embryos and we now have assumed a complete series of embryos from the first through the third trimester of gestation. Our proposed studies include and analysis of the development of sensory innervation of glabrous and hairy skin of the lip to compare with previous studies in other sites. We will compare the sequence of development in monkey with more limited human autopsy material and a series of opossum postnatal pups, the only non-primate animal with papillary ridges in palmar and plantar glabrous skin. We will compare the sequence of maturation of Merkel, Ruffini, Meissner, lanceolate and intraepidermal and dermal free nerve endings in glabrous as compared to hairy skin. Our completed baseline studies on the development of sensory innervation in hairy and glabrous monkey skin suggested a tight linkage between cutaneous and neural development, and prompted pilot studies on chick embryos and opossum pups following selective lesions of the neural tube. We propose to use the opossum, Monodelphis domesticus, as our experimental model in which we will analyze the sequential changes of cutaneous innervation following nerve transection and nerve crush in intact animals and in animals following partial abalation of the spinal cord. Altered cutaneous development, hyperplasia of residual dorsal root ganglia, and the expansion of the dermatotopic map following partial neuralectomy will be compared in Monodelphis to previous studies on chicken embryos. We will continue to analyze specimens of human skin following nerve transection and crush to better understand the residual sensibility following partial deafferentiation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS019462-04A1
Application #
3399511
Study Section
Communication Sciences and Disorders (CMS)
Project Start
1983-12-01
Project End
1990-03-31
Budget Start
1987-04-01
Budget End
1988-03-31
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Pennsylvania State University
Department
Type
Schools of Medicine
DUNS #
129348186
City
Hershey
State
PA
Country
United States
Zip Code
17033
Munger, B L; Bennett, G J; Kajander, K C (1992) An experimental painful peripheral neuropathy due to nerve constriction. I. Axonal pathology in the sciatic nerve. Exp Neurol 118:204-14
King, E D; Munger, B L (1990) Myotome and early neurogenesis in chick embryos. Anat Rec 228:191-210
Yoshida, Y; Ushiki, T; Takashio, M et al. (1989) Membrane relationships in murine Meissner corpuscles: cytology of freeze-substituted tissue. Anat Rec 223:437-45
Munger, B L; Renehan, W E (1989) Degeneration and regeneration of peripheral nerve in the rat trigeminal system: III. Abnormal sensory reinnervation of rat guard hairs following nerve transection and crush. J Comp Neurol 283:169-76
Ide, C; Yoshida, Y; Hayashi, S et al. (1988) A re-evaluation of the cytology of cat Pacinian corpuscles II. The extreme tip of the axon. Cell Tissue Res 253:95-103
Munger, B L; Ide, C (1988) The structure and function of cutaneous sensory receptors. Arch Histol Cytol 51:1-34
Munger, B L; Yoshida, Y; Hayashi, S et al. (1988) A re-evaluation of the cytology of cat Pacinian corpuscles. I. The inner core and clefts. Cell Tissue Res 253:83-93
Samulack, D D; Dykes, R W; Munger, B L (1988) Neurophysiologic aspects of allogeneic skin and upper extremity composite tissue transplantation in primates. Transplant Proc 20:279-90
Munger, B L; Ide, C (1987) The enigma of sensitivity in Pacinian corpuscles: a critical review and hypothesis of mechano-electric transduction. Neurosci Res 5:1-15
Ide, C; Nitatori, T; Munger, B L (1987) The cytology of human Pacinian corpuscles: evidence for sprouting of the central axon. Arch Histol Jpn 50:363-83

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