Abstract

The specific aims are to analyze mechanisms involved in normal and abnormal closure of the neural tube in mutant mice which exhibit neural dysraphism. Alterations in the cell surface and shape of neuroepithelial cells, as well as interactions between the neuroepithelial cells and adjacent chordamesodermal cells via the neural basal lamina will be studied by a variety of techniques, including immunocytochemistry and cytochemistry at the ultrastructural level, immunofluorescence histochemistry, scanning electron microscopy, and autoradiography. The expression of mutant genes in producing neural tube defects will be analyzed by low doses of retinoic acid, folic acid antagonists, and hydroxyurea. This project will help to contribute to an understanding of the etiology of specific human neurological disorders such as exencephaly, meningocele, anencephaly, and spina bifida.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS020055-06
Application #
3400259
Study Section
Neurology A Study Section (NEUA)
Project Start
1983-12-01
Project End
1990-11-30
Budget Start
1988-12-01
Budget End
1989-11-30
Support Year
6
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of California San Diego
Department
Type
Schools of Medicine
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093

  Publications

Wilson, D B; Wyatt, D P (1995) Immunofluorescent analysis of the spinal cord in dysraphic mice. Acta Anat (Basel) 154:120-7
Wilson, D B; Wyatt, D P (1995) Alterations in cranial morphogenesis in the Lp mutant mouse. J Craniofac Genet Dev Biol 15:182-9
Wilson, D B; Wyatt, D P (1995) Patterns of lectin binding during mammalian neurogenesis. J Anat 186 ( Pt 1):209-16
Wilson, D B; Wyatt, D P (1994) Analysis of neurulation in a mouse model for neural dysraphism. Exp Neurol 127:154-8
Wilson, D B; Wyatt, D P (1993) In vitro expression of neural tube pathology in the vl mutant mouse. J Neuropathol Exp Neurol 52:253-9
Wilson, D B; Wyatt, D P (1992) Aberrant convergence of the neural folds in the mouse mutant vl. Teratology 45:105-12
Wilson, D B; Wyatt, D P (1992) Abnormal elevation of the neural folds in the loop-tail mutant mouse. Acta Anat (Basel) 143:89-95
Wilson, D B; Wyatt, D P (1991) Ultrastructural analysis of the midaxial extracellular matrix in spinal dysraphism. Virchows Arch B Cell Pathol Incl Mol Pathol 60:113-8
Wilson, D B; Wyatt, D P; Gookin, J L (1990) Cranial effects of retinoic acid in the loop-tail (Lp) mutant mouse. J Craniofac Genet Dev Biol 10:75-81
Wilson, D B; Wyatt, D P (1989) Axial interactions during neurogenesis in dysraphic mice. Anat Embryol (Berl) 180:515-9

Showing the most recent 10 out of 21 publications