We have recently discovered a method to increase the absorption into draining lymph nodes of materials injected into the peritoneal cavity of rats. Applying this method to the study of experimental allergic encephalomyelitis (EAE) has enabled us to produce four new variants of this autoimmune disease: spastic, superacute, chronic demyelinating, and relapsing.
Our aim i s to establish the basic experimental requirements for uniform production of each of the new variants. The various patterns of localization of superacute EAE in the CNS will be studied, and an attempt will be made to control the distribution observed. The natural history of the chronic demyelinating and relapsing variants must be determined, and evidence of remyelination sought. In each instance, the putative relation between excessive absorption of antigenic inoculum and the clinical and pathologic peculiarities of the new EAE variants will be examined critically. These new procedures also offer unique opportunities to study the roles, if any, of CNS antigens other than myelin basic protein in chronicity, as well as the role of the antigenic depot in chronicity and relapses. Spasticity, chronicity, demyelination and relapses are important aspects of multiple sclerosis. Hyperacute or superacute syndromes occur in demyelinating diseases related to multiple sclerosis. Therefore, the results of this research should be highly relevant to multiple sclerosis. The availability of new forms of EAE and a better understanding of the mechanisms of their development should improve the utility of EAE as a model for multiple sclerosis, with respect to both pathogenesis and evaluation of proposed treatments.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS022261-02
Application #
3404477
Study Section
Neurology C Study Section (NEUC)
Project Start
1986-12-01
Project End
1989-11-30
Budget Start
1987-12-01
Budget End
1988-11-30
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost
Name
New York Medical College
Department
Type
Schools of Medicine
DUNS #
City
Valhalla
State
NY
Country
United States
Zip Code
10595
Levine, S; Saltzman, A (1991) Induction of arthritis in rats by aqueous suspensions of mycobacteria without the use of oil. Arthritis Rheum 34:63-7
Levine, S; Saltzman, A (1991) Anaphylaxis is enhanced by increased absorption of antigen in postinflammatory state. Int Arch Allergy Appl Immunol 95:207-11
Levine, S; Saltzman, A (1991) Tin compounds inhibit the plasma cell response to metallic tin. Transfer of inhibition by parabiosis. Biol Trace Elem Res 28:165-72
Levine, S; Saltzman, A (1991) Accelerated response to reinoculation in experimental allergic encephalomyelitis: histopathologic study. J Neuropathol Exp Neurol 50:126-33
Levine, S; Saltzman, A; Deibler, G E (1990) Peptides of myelin basic protein are encephalitogenic in rats without the aid of emulsions. Proc Soc Exp Biol Med 195:324-8
Levine, S; Saltzman, A; Deibler, G E (1990) Encephalitogenicity for rats of myelin basic protein without the aid of water-in-oil emulsions. J Neuropathol Exp Neurol 49:480-5
Levine, S; Saltzman, A; Loud, A (1989) Inflammatory siderosis of the nervous system in rats. J Neuropathol Exp Neurol 48:391-8
Levine, S; Saltzman, A (1989) Suppression of experimental allergic encephalomyelitis in rats by mercuric chloride. Chem Biol Interact 69:17-21
Levine, S; Saltzman, A (1989) The hyperacute form of allergic encephalomyelitis produced in rats without the aid of pertussis vaccine. J Neuropathol Exp Neurol 48:255-62
Levine, S; Saltzman, A (1988) The role of antigen absorption in the resistance of brown-Norway rats to experimental allergic encephalomyelitis. Immunol Lett 19:103-8

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