Chemical stimuli in the nasal cavity can be detected via olfactory, vomeronasal, and trigeminal receptors. Often, however, trigeminal chemoreception is overlooked by investigators in the chemical senses. Hence, behaviors and other effects of odors attributed primarily to olfactory or vomeronasal stimulation may in fact have a significant trigeminal component. Although trigeminal nerve endings in the nasal cavity respond to chemical stimuli, especially irritants, the nature of this response as well as the morphology of the receptors are not well characterized. Few data are available regarding the kinds and concentrations of compounds which stimulate trigeminal chemoreceptors. In addition, little is known about the location and ultrastructure of trigeminal chemoreceptors in the nasal cavity. The proposed research will examine the physiology and morphology of nasal trigeminal chemoreceptors. Single and multiunit recordings will be obtained from the ethmoid branch of the trigeminal nerve in the rat. Stimulus-response functions, threshold ranges, and latencies will be derived from a structurally diverse range of odorants, some commonly used in olfactory research, others obvious pollutants. Analysis also will focus on whether adaptation differs for different odorants. Cross adaptation experiments will examine the specificity of trigeminal chemoreceptors, i.e., can trigeminal chemoreceptors discriminate different odorants? Capsaicin desensitization, which depletes substance P and other neuropeptides, eliminates trigeminal responses to odorants. Therefore, light and high voltage electron microscopic immunocytochemical techniques will be used to identify the capsaicin-sensitive neuropeptide-containing trigeminal fibers and endings in the nasal epithelium. The distribution and ultrastructure of these neuropeptide-containing fibers will be determined.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS023326-03
Application #
3563729
Study Section
Communication Sciences and Disorders (CMS)
Project Start
1985-08-01
Project End
1988-07-31
Budget Start
1987-08-01
Budget End
1988-07-31
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Wake Forest University Health Sciences
Department
Type
DUNS #
041418799
City
Winston-Salem
State
NC
Country
United States
Zip Code
27106
Babensee, J E; McIntire, L V; Mikos, A G (2000) Growth factor delivery for tissue engineering. Pharm Res 17:497-504
Papadaki, M; Eskin, S G; Ruef, J et al. (1999) Fluid shear stress as a regulator of gene expression in vascular cells: possible correlations with diabetic abnormalities. Diabetes Res Clin Pract 45:89-99
Papadaki, M; Tilton, R G; Eskin, S G et al. (1998) Nitric oxide production by cultured human aortic smooth muscle cells: stimulation by fluid flow. Am J Physiol 274:H616-26
Papadaki, M; Ruef, J; Nguyen, K T et al. (1998) Differential regulation of protease activated receptor-1 and tissue plasminogen activator expression by shear stress in vascular smooth muscle cells. Circ Res 83:1027-34
Papadaki, M; Eskin, S G (1997) Effects of fluid shear stress on gene regulation of vascular cells. Biotechnol Prog 13:209-21
Yun, Z; Smith, T W; Menter, D G et al. (1997) Differential adhesion of metastatic RAW117 large-cell lymphoma cells under static or hydrodynamic conditions: role of integrin alpha(v) beta3. Clin Exp Metastasis 15:3-11
McIntire, L V (1994) 1992 ALZA Distinguished Lecture: bioengineering and vascular biology. Ann Biomed Eng 22:2-13
Silver, W L; Farley, L G; Finger, T E (1991) The effects of neonatal capsaicin administration on trigeminal nerve chemoreceptors in the rat nasal cavity. Brain Res 561:212-6
Finger, T E; Womble, M; Kinnamon, J C et al. (1990) Synapsin I-like immunoreactivity in nerve fibers associated with lingual taste buds of the rat. J Comp Neurol 292:283-90
Finger, T E; St Jeor, V L; Kinnamon, J C et al. (1990) Ultrastructure of substance P- and CGRP-immunoreactive nerve fibers in the nasal epithelium of rodents. J Comp Neurol 294:293-305

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