The in vivo panoramic imaging system developed during the original grant provides continuous brain PHi, cerebral blood flow, and NADH fluorescence data of the majority of the exposed cortex in the animal model used in this research. This imaging technology has facilitated consistent identification and characterization of the ischemic penumbra, the border zone region of reduced blood flow surrounding the core of dense ischemia or evolving infarction. As described in the Background Data, we have identified acidic foci within the ischemic penumbra. In an evolving infarction, it is postulated that part of the ischemic penumbra deteriorates leading to an increase in the size of the infarction. One of the hypothesis in this grant is that these acidic foci may play a critical role in the recruitment of the ischemic penumbra into infarction. Therefore, comprehending the pathophysiology of these acidic foci may lead to treatments which can reverse this process thereby reducing the degree of ischemic neuronal injury. The experiments outlined in this grant will first study whether the development of the acidic foci is initially due to perfusion or metabolic disturbances. It will then be detained whether enlargement of the infarction is due to expansion and coalescing of the acidic foci or rather expansion of the infarction at the periphery. The remainder of the experiments will examine the effect of various factors on the acidic foci including serum glucose concentrations, P0O2, systemic and extracellular brain pM, reperfusion, and brain temperature.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS025374-05A3
Application #
2265540
Study Section
Neurology A Study Section (NEUA)
Project Start
1988-02-15
Project End
1997-07-31
Budget Start
1994-08-17
Budget End
1995-07-31
Support Year
5
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
City
Rochester
State
MN
Country
United States
Zip Code
55905
Iuliano, B A; Anderson, R E; Meyer, F B (1995) Effect of intermittent reperfusion and nitric oxide synthase inhibition on infarct volume during reversible focal cerebral ischemia. J Neurosurg 83:491-5
Helgason, C M; Hoff, J A; Kondos, G T et al. (1993) Platelet aggregation in patients with atrial fibrillation taking aspirin or warfarin. Stroke 24:1458-61
Goldman, M S; Anderson, R E; Meyer, F B (1992) Effects of intermittent reperfusion during temporal focal ischemia. J Neurosurg 77:911-6
Sundt Jr, T M; Piepgras, D G; Stevens, L N (1991) Surgery for supratentorial arteriovenous malformations. Clin Neurosurg 37:49-115
Lyons, M K; Anderson, R E; Meyer, F B (1991) Basic fibroblast growth factor promotes in vivo cerebral angiogenesis in chronic forebrain ischemia. Brain Res 558:315-20
Sundt Jr, T M; Nichols, D A; Piepgras, D G et al. (1991) Strategies, techniques, and approaches for dural arteriovenous malformations of the posterior dural sinuses. Clin Neurosurg 37:155-70
Sundt Jr, T M; Piepgras, D G; Fode, N C et al. (1991) Giant intracranial aneurysms. Clin Neurosurg 37:116-54
Meyer, F B; Anderson, R E; Sundt Jr, T M (1990) Anticonvulsant effects of dihydropyridine Ca2+ antagonists in electrocortical shock seizures. Epilepsia 31:68-74
Sundt Jr, T M; Whisnant, J P; Houser, O W et al. (1990) Prospective study of the effectiveness and durability of carotid endarterectomy. Mayo Clin Proc 65:625-35
Atkinson, J L; Anderson, R E; Sundt Jr, T M (1990) The effect of carbon dioxide on the diameter of brain capillaries. Brain Res 517:333-40

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