Abstract

Regional blood flow to the brain changes with neural activity. We want to measure directly the blood flow in cerebral microvessels in the whisker barrel cortex of mice and rats. We are testing the hypothesis that the initial vasodilation and increased blood flow in the cerebral vasculature during natural activation of the brain is prompt and is localized in a small volume of the brain. The barrel cortex provides an ideal context for these studies because its neurophysiology, neuroanatomy, metabolism, and vascular architecture are well known. Video microscopy with image analysis will be used to record and measure the velocities of intravascular fluorescent beads and vessel diameters with fluorescent plasma markers. Laser Doppler flowmetry will provide convenient independent estimates of changes in local blood volume, velocity, and flow. Both assays will be used to evaluate the properties of the cortical vascular bed before, during, and after stimulation of the contralateral whiskers.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS028781-03
Application #
3415423
Study Section
Neurology A Study Section (NEUA)
Project Start
1990-09-21
Project End
1993-08-31
Budget Start
1992-09-01
Budget End
1993-08-31
Support Year
3
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Jost, Sarah C; Wanebo, John E; Song, Sheng-Kwei et al. (2007) In vivo imaging in a murine model of glioblastoma. Neurosurgery 60:360-70;discussion 370-1
Fox Jr, Douglas J; Velde, Hana Tysver; Preza, Chrysanthe et al. (2006) Computational hyperspectral interferometry for studies of brain function: proof of concept. Appl Opt 45:3009-21
Boero, J; Qin, W; Cheng, J et al. (2003) Restricted neuronal expression of ubiquitous mitochondrial creatine kinase: changing patterns in development and with increased activity. Mol Cell Biochem 244:69-76
Erinjeri, Joseph P; Woolsey, Thomas A (2002) Spatial integration of vascular changes with neural activity in mouse cortex. J Cereb Blood Flow Metab 22:353-60
Wei, L; Erinjeri, J P; Rovainen, C M et al. (2001) Collateral growth and angiogenesis around cortical stroke. Stroke 32:2179-84
Carmichael, S T; Wei, L; Rovainen, C M et al. (2001) New patterns of intracortical projections after focal cortical stroke. Neurobiol Dis 8:910-22
Boero, J A; Ascher, J; Arregui, A et al. (1999) Increased brain capillaries in chronic hypoxia. J Appl Physiol 86:1211-9
Sherburn, E W; Wanebo, J E; Kim, P et al. (1999) Gliomas in rodent whisker barrel cortex: a new tumor model. J Neurosurg 91:814-21
Moskalenko YuE; Rovainen, C; Woolsey, T A et al. (1996) Comparison of measurements of local brain blood flow by hydrogen clearance with the inhalation of hydrogen and its electrochemical generation in brain tissue. Neurosci Behav Physiol 26:245-50
Moskalenko, Y E; Dowling, J L; Liu, D et al. (1996) LCBF changes in rat somatosensory cortex during whisker stimulation monitored by dynamic H2 clearance. Int J Psychophysiol 21:45-59

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