Abstract

The theme of this application is that 13C and 15N NMR, including indirect detection methods, will be used to quantify the rates of glutamate and glutamine release and uptake in the rat brain in vivo. Studies will also be performed under selected conditions which were chosen for possible clinical relevance: hyperammonemia, steroid therapy, and after inhibition of the glutarnate transporter by an antisense oligonucleotide.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
3R01NS029048-05S1
Application #
6054576
Study Section
Biophysical Chemistry Study Section (BBCB)
Program Officer
Heemskerk, Jill E
Project Start
1994-08-01
Project End
2000-05-31
Budget Start
1998-06-01
Budget End
1999-05-31
Support Year
5
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Huntington Medical Research Institutes
Department
Type
DUNS #
077978898
City
Pasadena
State
CA
Country
United States
Zip Code
91101

  Publications

Kanamori, Keiko; Ross, Brian D (2004) Quantitative determination of extracellular glutamine concentration in rat brain, and its elevation in vivo by system A transport inhibitor, alpha-(methylamino)isobutyrate. J Neurochem 90:203-10
Kanamori, K; Kondrat, R W; Ross, B D (2003) 13C enrichment of extracellular neurotransmitter glutamate in rat brain--combined mass spectrometry and NMR studies of neurotransmitter turnover and uptake into glia in vivo. Cell Mol Biol (Noisy-le-grand) 49:819-36
Kondrat, Richard W; Kanamori, Keiko; Ross, Brian D (2002) In vivo microdialysis and gas-chromatography/mass-spectrometry for 13C-enrichment measurement of extracellular glutamate in rat brain. J Neurosci Methods 120:179-92
Kanamori, Keiko; Ross, Brian D; Kondrat, Richard W (2002) Glial uptake of neurotransmitter glutamate from the extracellular fluid studied in vivo by microdialysis and (13)C NMR. J Neurochem 83:682-95
Kanamori, K; Ross, B D (2001) The first in vivo observation of (13)C-(15)N coupling in mammalian brain. J Magn Reson 153:193-202
Kanamori, K; Ross, B D (1999) In vivo detection of (15)N-coupled protons in rat brain by ISIS localization and multiple-quantum editing. J Magn Reson 139:240-9
Kanamori, K; Ross, B D (1999) Localized 15N NMR spectroscopy of rat brain by ISIS. Magn Reson Med 41:456-63
Kanamori, K; Ross, B D; Kondrat, R W (1998) Rate of glutamate synthesis from leucine in rat brain measured in vivo by 15N NMR. J Neurochem 70:1304-15
Bluml, S; Zuckerman, E; Tan, J et al. (1998) Proton-decoupled 31P magnetic resonance spectroscopy reveals osmotic and metabolic disturbances in human hepatic encephalopathy. J Neurochem 71:1564-76
Kanamori, K; Ross, B D (1997) Glial alkalinization detected in vivo by 1H-15N heteronuclear multiple-quantum coherence-transfer NMR in severely hyperammonemic rat. J Neurochem 68:1209-20

Showing the most recent 10 out of 14 publications