This is an application to investigate glucose effects on memory in rodents. Using injections directly into the medial septum, amygdala and hippocampus, the first set of experiments determines which drug effects on learning and memory are sensitive to additional direct brain injections of glucose. Using intraseptal drug injections and microdialysis/HPLC measures of acetylcholine release in the hippocampal formation, the second set of experiments determines whether, regardless of the primary target of glucose actions, an output which predicts drug effects on learning and memory is release of acetylcholine. A third set of experiments employs determines whether 2-deoxyglucose uptake in the amygdala is depressed by intra-amygdala injections of morphine, muscimol, scopolamine, propranolol or AP5 and restored only in the first two instances (paralleling known and predicted behavioral results) by additional treatment with glucose. A fourth set of experiments determines whether, like glucose and glucose metabolites can attenuate memory impairments after intraseptal morphine injections. These findings provide initial evidence regarding the contribution of glucose metabolism to glucose effects on learning and memory.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS032914-04
Application #
2431235
Study Section
Special Emphasis Panel (SRC (22))
Program Officer
Broman, Sarah H
Project Start
1994-06-01
Project End
1999-05-31
Budget Start
1997-06-01
Budget End
1999-05-31
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Virginia
Department
Psychology
Type
Schools of Arts and Sciences
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Chang, Qing; Gold, Paul E (2008) Age-related changes in memory and in acetylcholine functions in the hippocampus in the Ts65Dn mouse, a model of Down syndrome. Neurobiol Learn Mem 89:167-77
McNay, Ewan C; Canal, Clinton E; Sherwin, Robert S et al. (2006) Modulation of memory with septal injections of morphine and glucose: effects on extracellular glucose levels in the hippocampus. Physiol Behav 87:298-303
Pych, Jason C; Kim, Munsoo; Gold, Paul E (2006) Effects of injections of glucose into the dorsal striatum on learning of place and response mazes. Behav Brain Res 167:373-8
Canal, Clinton E; McNay, Ewan C; Gold, Paul E (2005) Increases in extracellular fluid glucose levels in the rat hippocampus following an anesthetic dose of pentobarbital or ketamine-xylazine: an in vivo microdialysis study. Physiol Behav 84:245-50
Canal, Clinton E; Stutz, Sonja J; Gold, Paul E (2005) Glucose injections into the dorsal hippocampus or dorsolateral striatum of rats prior to T-maze training: modulation of learning rates and strategy selection. Learn Mem 12:367-74
Salinas, Juan A; Gold, Paul E (2005) Glucose regulation of memory for reward reduction in young and aged rats. Neurobiol Aging 26:45-52
Chang, Qing; Gold, Paul E (2004) Impaired and spared cholinergic functions in the hippocampus after lesions of the medial septum/vertical limb of the diagonal band with 192 IgG-saporin. Hippocampus 14:170-9
Chang, Qing; Gold, Paul E (2004) Inactivation of dorsolateral striatum impairs acquisition of response learning in cue-deficient, but not cue-available, conditions. Behav Neurosci 118:383-8
Gold, Paul E (2004) Coordination of multiple memory systems. Neurobiol Learn Mem 82:230-42
Savage, Lisa M; Chang, Qing; Gold, Paul E (2003) Diencephalic damage decreases hippocampal acetylcholine release during spontaneous alternation testing. Learn Mem 10:242-6

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