In this renewal application I propose experiments to study how a neuroinvasive alpha herpesvirus (pseudorabies virus, PRV) infection drives axonal targeting of virions and viral proteins to promote anterograde spread of infection in the nervous system. These experiments take advantage of our technology advances in imaging, neuronal culturing systems, mass spectrometry, and deep sequencing, as well as new findings from the last funding period. In particular, we will focus on imaging US9, a critical protein for sorting virions into axons (aim 1). Using mass spectrometry and new enrichment techniques, we will determine the proteome of enriched vesicles containing GFP-US9 and gE-GFP and initiate studies to identify US9-specific protein complexes (aim 2). Finally, we will extend our work to test new hypotheses concerning the axonal damage response, the role of local axonal translation on viral axonal transport, and, using deep sequencing technology (Ilumina platform), how PRV infection affects not only axonal targeting of viral proteins, but also of host and viral mRNAs (aim 3).

Public Health Relevance

The direction taken by the virus in a neuron after primary infection or reactivation from latency, as well as the extent of spread in a neuronal circuit can be the difference between a minor peripheral infection and lethal brain infection. My work will provide basic knowledge toward understanding how to block neuronal spread and its resulting damage. As viral tracing of neural circuitry has become an essential tool in the neuroscience community, our new discoveries will be immediately applicable for many ongoing fundamental research projects in neuroscience in a variety of animals with the promise to reveal detailed functional insights into neural circuit organization that have not been possible to achieve in the past.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Project (R01)
Project #
Application #
Study Section
Virology - A Study Section (VIRA)
Program Officer
Wong, May
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Princeton University
Schools of Arts and Sciences
United States
Zip Code
Hogue, Ian B; Card, J Patrick; Rinaman, Linda et al. (2018) Characterization of the neuroinvasive profile of a pseudorabies virus recombinant expressing the mTurquoise2 reporter in single and multiple injection experiments. J Neurosci Methods 308:228-239
Hogue, Ian B; Jean, Jolie; Esteves, Andrew D et al. (2018) Functional Carboxy-Terminal Fluorescent Protein Fusion to Pseudorabies Virus Small Capsid Protein VP26. J Virol 92:
Koyuncu, Orkide O; MacGibeny, Margaret A; Enquist, Lynn W (2018) Latent versus productive infection: the alpha herpesvirus switch. Future Virol 13:431-443
Harris, Greg M; Madigan, Nicolas N; Lancaster, Karen Z et al. (2017) Nerve Guidance by a Decellularized Fibroblast Extracellular Matrix. Matrix Biol 60-61:176-189
Enquist, Lynn W; Leib, David A (2017) Intrinsic and Innate Defenses of Neurons: D├ętente with the Herpesviruses. J Virol 91:
Bosse, Jens B; Enquist, Lynn W (2016) The diffusive way out: Herpesviruses remodel the host nucleus, enabling capsids to access the inner nuclear membrane. Nucleus 7:13-9
Song, Ren; Koyuncu, Orkide O; Greco, Todd M et al. (2016) Two Modes of the Axonal Interferon Response Limit Alphaherpesvirus Neuroinvasion. MBio 7:e02145-15
Johnson, Blake N; Lancaster, Karen Z; Hogue, Ian B et al. (2016) 3D printed nervous system on a chip. Lab Chip 16:1393-400
Kratchmarov, R; Enquist, L W; Taylor, M P (2015) Us9-Independent Axonal Sorting and Transport of the Pseudorabies Virus Glycoprotein gM. J Virol 89:6511-4
Bosse, Jens B; Hogue, Ian B; Feric, Marina et al. (2015) Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion. Proc Natl Acad Sci U S A 112:E5725-33

Showing the most recent 10 out of 60 publications