The overall goal of this proposal is to test the hypothesis that astrocytes modulate synaptic transmission. Previous work from the applicant's laboratory has shown that calcium elevations in astrocytes lead to delayed calcium elevation in adjacent neurons. In this proposal, the applicant will address three tightly interrelated hypothesis designed to examine the role of astrocytes. First, he will test the hypothesis that Ca++ dependent glutamate release from astrocytes requires the SNARE fusion complex. Second, he will test the hypothesis that activation of astrocytes causes mGluR and NMDA receptor dependent synaptic modulation. Finally, he will test the hypothesis that astrocytes regulate synaptic transmission by controlling intracellular calcium levels in presynaptic terminals. If successful, these experiments will provide solid information on the role of astrocytes in synaptic transmission.
| Papouin, Thomas; Haydon, Philip G (2018) D-serine Measurements in Brain Slices or Other Tissue Explants. Bio Protoc 8: |
| Papouin, Thomas; Haydon, Philip G (2018) Obtaining Acute Brain Slices. Bio Protoc 8: |
| Haydon, Philip G (2017) Astrocytes and the modulation of sleep. Curr Opin Neurobiol 44:28-33 |
| Clasadonte, Jerome; Scemes, Eliana; Wang, Zhongya et al. (2017) Connexin 43-Mediated Astroglial Metabolic Networks Contribute to the Regulation of the Sleep-Wake Cycle. Neuron 95:1365-1380.e5 |
| Foley, Jeannine; Blutstein, Tamara; Lee, SoYoung et al. (2017) Astrocytic IP3/Ca2+Signaling Modulates Theta Rhythm and REM Sleep. Front Neural Circuits 11:3 |
| Papouin, Thomas; Dunphy, Jaclyn; Tolman, Michaela et al. (2017) Astrocytic control of synaptic function. Philos Trans R Soc Lond B Biol Sci 372: |
| Keck, Tara; Toyoizumi, Taro; Chen, Lu et al. (2017) Integrating Hebbian and homeostatic plasticity: the current state of the field and future research directions. Philos Trans R Soc Lond B Biol Sci 372: |
| Papouin, Thomas; Dunphy, Jaclyn M; Tolman, Michaela et al. (2017) Septal Cholinergic Neuromodulation Tunes the Astrocyte-Dependent Gating of Hippocampal NMDA Receptors to Wakefulness. Neuron 94:840-854.e7 |
| Clasadonte, Jerome; Morel, Lydie; Barrios-Camacho, Camila M et al. (2016) Molecular analysis of acute and chronic reactive astrocytes in the pilocarpine model of temporal lobe epilepsy. Neurobiol Dis 91:315-25 |
| Haydon, Philip G (2016) The Evolving View of Astrocytes. Cerebrum 2016: |
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