The reticulospinal system (RS) is a major descending motor control system. It has not been possible to specifically stimulate or lesion discrete functional subsets of RS neurons and so our understanding of RS signals being transmitted to the spinal cord is poorly understood. The transparent hindbrain of the larval Zebrafish which is accessible to optical techniques solves this problem. Further, the larvae's RS system is quite simple. It is comprised of about 100 neurons, most of which can be identified in the living fish. This allows both optical recording of neural activity and laser ablation of either individual neurons or subsets of neurons. Further, given the small total number of neurons, it is feasible to make precise ablations and quantify the effects on such larval behavior as swimming, escaping, feeding and more complex behaviors. Thus, the larval Zebrafish hindbrain provides an ideal opportunity for understanding the neural control of different behaviors.
The first aim i s to ablate all RS neurons to delimit the range of behaviors that the RS system mediates.
The second aim will use optical recording and laser ablation to identify which specific RS neurons are involved in which locomotor behaviors. This will not only reveal the functional organization of the Zebrafish hindbrain but allow the evaluation of several competing hypotheses on the forms of network organization used to implement motor control.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS037789-02
Application #
2892439
Study Section
Neurology B Subcommittee 2 (NEUB)
Program Officer
Michel, Mary E
Project Start
1998-09-15
Project End
2002-08-31
Budget Start
1999-09-01
Budget End
2000-08-31
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Northeastern University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
039318308
City
Boston
State
MA
Country
United States
Zip Code
02115
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