The peripheral sensory nervous system in the vertebrate head has a dull origin from cranial ectodermal placodes and neural crest cells. Placodes are discrete regions of thickened columnar epithelium on the head that share many properties with neural crest cells including the ability to undergo an epithelial to mesenchymal transition and migrate. Although the neural crest is being studied in depth, far less in known about placodes. We will explore the tissue and molecular interations that lead to placode formation and will use molecular markers for specific placodes to assess their origin and differentiation. We will primarily concentrate on the trigeminal placode, which develops adjacent to the neural tube, and the nodose placode, which develops at some distance from the neural tube. In addition, molecular markers for other placodes will be explored. An important component of this study will be to explore intrinsic similarities and differences between placodes and neural crest, in order to shed light on their embryonic relationships. Specifically, we will examine when placode and neural crest cell lineages segregate during development by determining how late in development a single cell can contribute to both placode and neural crest cell linages. We will study whether and to what extent placodes can form neural crest cell derivatives by examining their ability to interchange for one another. Finally, we will explore why the rostral neural folds from placodes but not neural crest cells. Specific experiments will: 1) examine the induction and development of the trigeminal placode. 2) examine the induction and development of the nodose placode. 3) identify molecular markers for other epibranchial placodes. 4) examine the interrelationships of the neural crest and placodes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
1R01NS041070-01
Application #
6175937
Study Section
Special Emphasis Panel (ZRG1-MDCN-6 (01))
Program Officer
Finkelstein, Robert
Project Start
2000-08-20
Project End
2004-05-31
Budget Start
2000-08-20
Budget End
2001-05-31
Support Year
1
Fiscal Year
2000
Total Cost
$376,616
Indirect Cost
Name
California Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
078731668
City
Pasadena
State
CA
Country
United States
Zip Code
91125
Shiau, Celia E; Hu, Na; Bronner-Fraser, Marianne (2010) Altering Glypican-1 levels modulates canonical Wnt signaling during trigeminal placode development. Dev Biol 348:107-18
Ramesh, Aramandla; Inyang, Frank; Lunstra, Donald D et al. (2008) Alteration of fertility endpoints in adult male F-344 rats by subchronic exposure to inhaled benzo(a)pyrene. Exp Toxicol Pathol 60:269-80
Lee, Vivian M; Bronner-Fraser, Marianne; Baker, Clare V H (2005) Restricted response of mesencephalic neural crest to sympathetic differentiation signals in the trunk. Dev Biol 278:175-92
Bhattacharyya, Sujata; Bailey, Andrew P; Bronner-Fraser, Marianne et al. (2004) Segregation of lens and olfactory precursors from a common territory: cell sorting and reciprocity of Dlx5 and Pax6 expression. Dev Biol 271:403-14
Venters, Sara J; Argent, Rebecca E; Deegan, Fiona M et al. (2004) Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling. Dev Dyn 229:591-9
Inyang, Frank; Ramesh, Aramandla; Kopsombut, Prapaporn et al. (2003) Disruption of testicular steroidogenesis and epididymal function by inhaled benzo(a)pyrene. Reprod Toxicol 17:527-37
Lee, Vivian M; Sechrist, John W; Luetolf, Simone et al. (2003) Both neural crest and placode contribute to the ciliary ganglion and oculomotor nerve. Dev Biol 263:176-90
Lee, Vivian M; Sechrist, John W; Bronner-Fraser, Marianne et al. (2002) Neuronal differentiation from postmitotic precursors in the ciliary ganglion. Dev Biol 252:312-23
Kious, Brent M; Baker, Clare V H; Bronner-Fraser, Marianne et al. (2002) Identification and characterization of a calcium channel gamma subunit expressed in differentiating neurons and myoblasts. Dev Biol 243:249-59
Baker, Clare V H; Stark, Michael R; Bronner-Fraser, Marianne (2002) Pax3-expressing trigeminal placode cells can localize to trunk neural crest sites but are committed to a cutaneous sensory neuron fate. Dev Biol 249:219-36

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