Understanding the biophysical properties of single neurons and how they process information is fundamental to understanding how brain works. The long-term objective of this proposal is to contribute to understanding the logic of information processing in the dendrites of individual vertebrate neurons. We will utilize new tools, based on a recently developed high-resolution imaging technique with intracellular voltage-sensitive dyes, to examine two notable spatial aspects of dendritic integration; (a) processing of sensory information in two functional compartments of mitral cells of the olfactory bulb and (b) functional subdivisions of CA1 pyramidal neuron dendritic tree. An accurate description of interactions between excitatory and inhibitory components in different dendritic compartments of mitral and tufted cells should facilitate understanding of the process of signal integration in the olfactory bulb. Such an understanding would be an important step toward comprehension of neuronal mechanisms involved in sensory perception. The postulate that synaptic amplification of the backpropagating action potential is restricted to the site of synaptic input is based on computer simulation and indirect experimental evidence. This is an important hypothesis because spatially restricted changes in excitability could functionally subdivide a neuron; multiple functional compartments, in turn, have important implications for synaptic integration and plasticity. The results of this analysis will bear on the basic neuroscience of signaling.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS042739-02
Application #
6620523
Study Section
Integrative, Functional and Cognitive Neuroscience 8 (IFCN)
Program Officer
Talley, Edmund M
Project Start
2001-12-15
Project End
2005-11-30
Budget Start
2002-12-01
Budget End
2003-11-30
Support Year
2
Fiscal Year
2003
Total Cost
$310,650
Indirect Cost
Name
Yale University
Department
Physiology
Type
Schools of Medicine
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Popovic, Marko A; Carnevale, Nicholas; Rozsa, Balazs et al. (2015) Electrical behaviour of dendritic spines as revealed by voltage imaging. Nat Commun 6:8436
Homma, R; Kovalchuk, Y; Konnerth, A et al. (2013) In vivo functional properties of juxtaglomerular neurons in the mouse olfactory bulb. Front Neural Circuits 7:23
Homma, Ryota; Baker, Bradley J; Jin, Lei et al. (2009) Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes. Philos Trans R Soc Lond B Biol Sci 364:2453-67
Homma, Ryota; Baker, Bradley J; Jin, Lei et al. (2009) Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes. Methods Mol Biol 489:43-79
Canepari, Marco; Vogt, Kaspar; Zecevic, Dejan (2008) Combining voltage and calcium imaging from neuronal dendrites. Cell Mol Neurobiol 28:1079-93
Canepari, Marco; Djurisic, Maja; Zecevic, Dejan (2007) Dendritic signals from rat hippocampal CA1 pyramidal neurons during coincident pre- and post-synaptic activity: a combined voltage- and calcium-imaging study. J Physiol 580:463-84
Popovic, Marko; Djurisic, Maja; Zecevic, Dejan (2005) Determinants of low EPSP attenuation in primary dendrites of mitral cells: modeling study. Ann N Y Acad Sci 1048:344-8
Baker, Bradley J; Kosmidis, Efstratios K; Vucinic, Dejan et al. (2005) Imaging brain activity with voltage- and calcium-sensitive dyes. Cell Mol Neurobiol 25:245-82