The long-term goal of this study is to understand the organization of cortical neuronal activity in sustained seizure events and in sensory processing. In this proposal, we will study a special form of population neuronal activity, namely, spiral waves. Spiral waves are a ubiquitous feature of excitable systems in nature, where they play a role in pattern formation and the organization of flow dynamics. Within biomedical science, spiral wave dynamics have been studied extensively in cardiac electrophysiology, and have greatly advanced our understanding of arrhythmogenic mechanisms. Surprisingly, however, only a few researchers have studied spiral dynamics in the brain. Following our recent discovery of spiral waves in rat neocortical slices, in this proposal, we will experimentally verify the existence of spiral waves in rodent neocortex in vivo during seizure-like events and during sensory evoked and spontaneous activity. Cortex in vivo has extensive long-range connections which are not present in brain slices. It is therefore necessary to experimentally examine the initiation and sustaining of spiral waves in intact cortex, given that strong long-range thalamocortical and corticocortical connections may disrupt the development of these spiral waves.
Three Specific Aims are proposed to study wave-to-wave interactions in rat sensory and motor cortices.
Aim 1 is devoted to improve voltage-sensitive dye imaging methods in order to identify phase singularities at the spiral center.
Aim 2 is to examine the incidence rate of spirals during seizure-like activity in various cortical areas.
Aim 3 is to investigate spiral dynamics during sensory-evoked activity and sleep-like waves. Studying spiral dynamics in the cortex will directly contribute to understanding of the initiation and sustaining of seizure activity. Spirals are known as a major contributor to arrhythmic activity in cardiac tissue, and extinguishing spirals in the heart has been a therapeutic strategy for preventing cardiac fibrillation. This project is highly relevant to the mission of the NINDS, and should contribute to the understanding of initiation and sustaining of epileptic activity in the cortex, which disturbs the life of about 1% of the US population. ? ? ?
| Geng, Xinling; Wu, Jian-Young (2017) 'Blue' voltage-sensitive dyes for studying spatiotemporal dynamics in the brain: visualizing cortical waves. Neurophotonics 4:031207 |
| Vitantonio, Daniel; Xu, Weifeng; Geng, Xinling et al. (2015) Emergence of dominant initiation sites for interictal spikes in rat neocortex. J Neurophysiol 114:3315-25 |
| Rozeboom, Aaron M; Queenan, Bridget N; Partridge, John G et al. (2015) Evidence for glycinergic GluN1/GluN3 NMDA receptors in hippocampal metaplasticity. Neurobiol Learn Mem 125:265-73 |
| Liang, Jianmin; Xu, Weifeng; Geng, Xinling et al. (2015) Monitoring Population Membrane Potential Signals from Neocortex. Adv Exp Med Biol 859:171-96 |
| Baker, Bradley; Gao, Xin; Wolff, Brian S et al. (2015) In Vivo Voltage-Sensitive Dye Imaging of Mammalian Cortex Using ""Blue"" Dyes. Cold Spring Harb Protoc 2015:1000-2 |
| Baker, Bradley; Gao, Xin; Wolff, Brian S et al. (2015) Voltage-Sensitive Dye Imaging of Population Signals in Brain Slices. Cold Spring Harb Protoc 2015:995-9 |
| Xu, Weifeng; Wolff, Brian S; Wu, Jian-young (2014) Low-intensity electric fields induce two distinct response components in neocortical neuronal populations. J Neurophysiol 112:2446-56 |
| Gao, X; Xu, W; Wang, Z et al. (2012) Interactions between two propagating waves in rat visual cortex. Neuroscience 216:57-69 |
| Takagaki, Kentaroh; Zhang, Chuan; Wu, Jian-Young et al. (2011) Flow detection of propagating waves with temporospatial correlation of activity. J Neurosci Methods 200:207-18 |
| Huang, Xiaoying; Xu, Weifeng; Liang, Jianmin et al. (2010) Spiral wave dynamics in neocortex. Neuron 68:978-990 |
