Excessive activation of the 1-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) subtype of ionotropic glutamate receptors has been implicated as a leading contributor to a number of neurological diseases, such as epilepsy, stroke and amyotrophic lateral sclerosis (ALS). Using inhibitors as neuroprotective drugs to dampen the excessive receptor activity has been a long pursued therapeutic strategy. 2,3-Benzodiazepine derivatives, also known as GYKI compounds, are inhibitors of AMPA receptors, and they represent a class of the most promising drug candidates developed to date. However, the quantitative, functional activities of these compounds on AMPA receptors remain poorly defined. This is because AMPA receptors open their channels in the microsecond time domain and desensitize even in the millisecond time region. Yet, current kinetic techniques do not have sufficient time resolutions required to characterize the kinetic mechanism of channel opening and the mechanism of inhibitor/drug- receptor interaction. In this proposal, we will systematically elucidate the mechanism of action for a series of 19 GYKI compounds, measure their potency on specific AMPA receptor subunits, and characterize the structure-activity relationship, including the number of inhibitory sites on a receptor and whether any two sites interact with each other (i.e., the binding of two inhibitors to their sites can be independent or negatively affected by binding of either one first). To achieve the specific aims, we will carry out a number of experiments, including a laser- pulse photolysis study with the ?s time resolution to investigate the effect of a GYKI compound on the channel-opening rate process of an AMPA receptor. The kinetic investigation of these compounds, relevant to the time scale within which all receptor forms are still functional, has not been previously possible. Our results on the receptor properties and the structure-activity relationship of these compounds will be valuable for rational design and synthesis of subunit- and conformation-selective GYKI compounds with higher potency so that AMPA receptor activities can be controlled more quantitatively.

Public Health Relevance

We propose to systematically characterize a group of GYKI compounds, which are inhibitors on AMPA glutamate ion channel receptors. These compounds are candidates for developing potential drugs to treat a number of neurological diseases involving AMPA receptors.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS060812-02
Application #
7782832
Study Section
Molecular Neuropharmacology and Signaling Study Section (MNPS)
Program Officer
Silberberg, Shai D
Project Start
2009-03-06
Project End
2014-02-28
Budget Start
2010-03-01
Budget End
2011-02-28
Support Year
2
Fiscal Year
2010
Total Cost
$292,160
Indirect Cost
Name
State University of New York at Albany
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
152652822
City
Albany
State
NY
Country
United States
Zip Code
12222
Wen, Wei; Lin, Chi-Yen; Niu, Li (2017) R/G editing in GluA2Rflop modulates the functional difference between GluA1 flip and flop variants in GluA1/2R heteromeric channels. Sci Rep 7:13654
Jaremko, William J; Huang, Zhen; Wen, Wei et al. (2017) Identification and characterization of RNA aptamers: A long aptamer blocks the AMPA receptor and a short aptamer blocks both AMPA and kainate receptors. J Biol Chem 292:7338-7347
Jaremko, William J; Huang, Zhen; Wen, Wei et al. (2017) One aptamer, two functions: the full-length aptamer inhibits AMPA receptors, while the short one inhibits both AMPA and kainate receptors. RNA Dis 4:
Huang, Zhen; Wen, Wei; Wu, Andrew et al. (2017) Chemically Modified, ?-Amino-3-hydroxy-5-methyl-4-isoxazole (AMPA) Receptor RNA Aptamers Designed for in Vivo Use. ACS Chem Neurosci 8:2437-2445
Wang, Congzhou; Wu, Andrew; Shen, Yu-Chuan et al. (2015) Mechanism and site of inhibition of AMPA receptors: substitution of one and two methyl groups at the 4-aminophenyl ring of 2,3-benzodiazepine and implications in the ""E"" site. ACS Chem Neurosci 6:1371-8
Lin, Chi-Yen; Huang, Zhen; Wen, Wei et al. (2015) Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature. PLoS One 10:e0123562
Niu, Li (2015) Mechanism-based design of 2,3-benzodiazepine inhibitors for AMPA receptors. Acta Pharm Sin B 5:500-5
Niu, Li (2014) AMPA receptor inhibitors for the treatment of neurological disorders. Future Med Chem 6:849-952
Lin, Chi-Yen; Huang, Zhen; Jaremko, William et al. (2014) High-performance liquid chromatography purification of chemically modified RNA aptamers. Anal Biochem 449:106-8
Wu, Andrew; Wang, Congzhou; Niu, Li (2014) Mechanism of inhibition of the GluA1 AMPA receptor channel opening by the 2,3-benzodiazepine compound GYKI 52466 and a N-methyl-carbamoyl derivative. Biochemistry 53:3033-41

Showing the most recent 10 out of 22 publications