Epidermal cells provide the first point of contact for sensory stimuli and are innervated by somatosensory neurons (SSNs) that shape our experience of the world. Both SSNs and epidermal cells are of great clinical relevance; SSNs are mediators of physiological and pathological pain, and some pathological skin conditions are associated with debilitating pain and itch. However, our understanding of roles that epidermal cells play in SSN development and function, particularly nociception, remain limited aside from a few well-studied examples. Characterizing these important intercellular interactions is complicated by the heterogeneity and complexity of vertebrate nervous systems. Drosophila provides an appealing system to close this gap in our knowledge, offering a wealth of genetic resources, ready imaging access of SSNs/epidermis with single cell resolution, a compact nervous system, and evolutionary conservation of key regulators of SSN development/function. In this project we will use an integrated approach to study an evolutionarily conserved intracellular interaction that involves the wrapping of SSN neurites by epidermal cells. The conservation of this intercellular interaction and the preferential ensheathment of nociceptors compared to other SSNs suggest that these sheaths may play key roles in development and function of nociceptive SSNs. Here, we test the hypothesis that epidermal ensheathment of SSNs functionally couples epidermal cells to SSNs. We will test this hypothesis using three lines of experimentation. First, we will characterize the response properties of Drosophila epidermal cells and identify epidermal sensory channels that mediate epidermal responses to noxious stimuli. Second, we will test requirements for sheaths in epidermal activation of nociceptors, define the neuronal substrates for epidermally-gated behavior responses, define the SSN repertoire functionally coupled to epidermal stimulation, and quantify contributions of epidermal activation to sensory-evoked behaviors. Third, we will identify signaling mechanisms linking epidermis and C4da neurons in the periphery and identify circuit- level effects of ensheathment. Given the enormous impact of pathological pain on quality of life ? chronic pain affects more Americans than diabetes, heart disease, and cancer combined ? understanding how epidermal cells modulate nociceptive SSN function is of great interest for development of novel therapeutics for pain management. Successful completion of this project will provide insight into a fundamental component of somatosensation that represents a novel control point for nociception that could define a new entry point for pain management.

Public Health Relevance

Twenty million Americans suffer from peripheral neuropathies, and one in three individuals in the U.S. will suffer from chronic pain. Somatosensory neurons (SSNs) are mediators of physiological and pathological pain, therefore understanding key control points for SSN development and function is of paramount importance for therapeutic intervention in this health crisis. This proposal tests the hypothesis that an evolutionarily conserved interaction between epidermal cells and nociceptive SSNs, epidermal neurite ensheathment, regulates the function of SSNs and therefore represents a novel control point for therapies designed to manage pain.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS076614-08
Application #
9824422
Study Section
Somatosensory and Pain Systems Study Section (SPS)
Program Officer
Lavaute, Timothy M
Project Start
2011-09-01
Project End
2024-06-30
Budget Start
2019-07-01
Budget End
2020-06-30
Support Year
8
Fiscal Year
2019
Total Cost
Indirect Cost
Name
University of Washington
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Hoyer, Nina; Zielke, Philip; Hu, Chun et al. (2018) Ret and Substrate-Derived TGF-? Maverick Regulate Space-Filling Dendrite Growth in Drosophila Sensory Neurons. Cell Rep 24:2261-2272.e5
Vinauger, Clément; Lahondère, Chloé; Wolff, Gabriella H et al. (2018) Modulation of Host Learning in Aedes aegypti Mosquitoes. Curr Biol 28:333-344.e8
Utashiro, Nao; Williams, Claire R; Parrish, Jay Z et al. (2018) Prior activity of olfactory receptor neurons is required for proper sensory processing and behavior in Drosophila larvae. Sci Rep 8:8580
Yan, Connie; Wang, Fei; Peng, Yun et al. (2018) Microtubule Acetylation Is Required for Mechanosensation in Drosophila. Cell Rep 25:1051-1065.e6
Jiang, Nan; Kim, Hyeon-Jin; Chozinski, Tyler J et al. (2018) Superresolution imaging of Drosophila tissues using expansion microscopy. Mol Biol Cell 29:1413-1421
Williams, Claire R; Baccarella, Alyssa; Parrish, Jay Z et al. (2017) Empirical assessment of analysis workflows for differential expression analysis of human samples using RNA-Seq. BMC Bioinformatics 18:38
Boiko, Nina; Medrano, Geraldo; Montano, Elizabeth et al. (2017) TrpA1 activation in peripheral sensory neurons underlies the ionic basis of pain hypersensitivity in response to vinca alkaloids. PLoS One 12:e0186888
Williams, Claire R; Baccarella, Alyssa; Parrish, Jay Z et al. (2016) Trimming of sequence reads alters RNA-Seq gene expression estimates. BMC Bioinformatics 17:103
Meltzer, Shan; Yadav, Smita; Lee, Jiae et al. (2016) Epidermis-Derived Semaphorin Promotes Dendrite Self-Avoidance by Regulating Dendrite-Substrate Adhesion in Drosophila Sensory Neurons. Neuron 89:741-55
Lee, Jiae; Peng, Yun; Lin, Wen-Yang et al. (2015) Coordinate control of terminal dendrite patterning and dynamics by the membrane protein Raw. Development 142:162-73

Showing the most recent 10 out of 15 publications