Unlike all other organs, the brain and spinal cord lack lymphatic vessels. Traditional thought has averred that the brain - despite having the highest basal metabolic rate of any organ - can function without such an organized network for the removal of interstitial fluid-borne metabolic waste products. We questioned this position, seeking to define the pathways by which the brain removes the potentially toxic byproducts of cellular activity. Our preliminary analysis, based on in vivo two-photon imaging, shows that low molecular weight tracers delivered to the CSF circulate surprisingly rapidly through the mouse brain, and do so along a defined anatomical route. This consists of a para-arterial inflow path, an intra-parenchymal path of interstitial flow, and a para-venous outflow path. Within the interstitial space, astrocytes support convective fluid currents, as deletion of the astrocytic watr channel AQP4 sharply reduces tracer flow along these routes. Given the continuous movement of fluid along this pathway, and its critical dependence upon astrocytic fluid transport, we propose that this system - which we designate the 'glymphatic system' - subserves a function homologous to the peripheral lymphatic system, and is essential for the clearance of metabolic waste products from the CNS. We will test the provocative hypothesis that cognitive function in an experimental model of vascular dementia in part is suppressed by accumulation of metabolic waste products. This hypothesis is based on the observation that glymphatic transport is sharply reduced in a murine model of multi-lacunar infarcts, which results in widespread trapping of small tracers in the lesioned hemisphere.
Aim 1 will use in vivo 2-photon microscopy to assess the spatial dynamics and temporal kinetics of fluorophore-tagged tracer clearance. By systematically comparing the effect of modifications of molecular sizes or surface charge upon tracer clearance, we will define the basic transport properties of the glymphatic system.
Aim 2 will extend the preliminary finding that aged mice exhibit a striking decline in glymphatic system function, and evaluate the effect that age-related suppression of arterial wall pulsation and resulting loss of convective inflow along the para-arterial path has on glymphatic function.
Aim 3 will extend the observation that intra-parenchymal fluid movement is reduced in a mouse model of multi-lacunar infarcts and evaluate whether aging cause an additional suppression of glymphatic clearance.
Aim 4 will take advantage of inducible astrocyte-specific deletion of AQP4 transgenic mice and test the hypothesis that suppressing glymphatic transport in mice with multi-lacunar infarcts will impair their cognitive functions independently of the ischemic injury. To our knowledge, these studies represent the first attempt to systematically define the mechanisms involved in the clearance of metabolic waste products from the brain on a whole-organ level. The proposed studies will provide fundamental new insight into cognitive impairment in vascular dementia, and will likely also improve our understanding of the pathophysiology of brain injury following stroke and head trauma.
|Smith, Nathan A; Kress, Benjamin T; Lu, Yuan et al. (2018) Fluorescent Ca2+ indicators directly inhibit the Na,K-ATPase and disrupt cellular functions. Sci Signal 11:|
|Ratner, Vadim; Gao, Yi; Lee, Hedok et al. (2017) Cerebrospinal and interstitial fluid transport via the glymphatic pathway modeled by optimal mass transport. Neuroimage 152:530-537|
|Wang, Minghuan; Ding, Fengfei; Deng, SaiYue et al. (2017) Focal Solute Trapping and Global Glymphatic Pathway Impairment in a Murine Model of Multiple Microinfarcts. J Neurosci 37:2870-2877|
|Wang, Fushun; Wang, Xiaowei; Shapiro, Lee A et al. (2017) NKCC1 up-regulation contributes to early post-traumatic seizures and increased post-traumatic seizure susceptibility. Brain Struct Funct 222:1543-1556|
|Achariyar, Thiyagaragan M; Li, Baoman; Peng, Weiguo et al. (2017) Erratum to: Glymphatic distribution of CSF-derived apoE into brain is isoform specific and suppressed during sleep deprivation. Mol Neurodegener 12:3|
|Lundgaard, Iben; Lu, Minh Lon; Yang, Ezra et al. (2017) Glymphatic clearance controls state-dependent changes in brain lactate concentration. J Cereb Blood Flow Metab 37:2112-2124|
|Cotrina, Maria L; Lou, Nanghong; Tome-Garcia, Jessica et al. (2017) Direct comparison of microglial dynamics and inflammatory profile in photothrombotic and arterial occlusion evoked stroke. Neuroscience 343:483-494|
|Sun, Wei; Cornwell, Adam; Li, Jiashu et al. (2017) SOX9 Is an Astrocyte-Specific Nuclear Marker in the Adult Brain Outside the Neurogenic Regions. J Neurosci 37:4493-4507|
|Jiang, Quan; Zhang, Li; Ding, Guangliang et al. (2017) Impairment of the glymphatic system after diabetes. J Cereb Blood Flow Metab 37:1326-1337|
|de Leon, Mony J; Li, Yi; Okamura, Nobuyuki et al. (2017) Cerebrospinal Fluid Clearance in Alzheimer Disease Measured with Dynamic PET. J Nucl Med 58:1471-1476|
Showing the most recent 10 out of 91 publications