This is a Multiple-PI R01 proposal for coordinated investigation of the striatum, a brain structure critically involved in normal movement and motivation. Altered striatal function underlies a range of serious, common neurological disorders, including Parkinson's Disease and dystonia. Yet the mechanisms by which this structure normally processes information, and how this can go awry, are not well understood. One particular cell type, the fast-spiking interneuron (FSI), is rare but has a disproportionate influence over other striatal neurons. Loss of FSIs has been observed in animal models of dystonia and in human Tourette syndrome. In recent studies we have observed activation of FSIs as highly trained yet unwanted choices need to be suppressed, and that selective suppression of FSIs results in dystonia-like symptoms. FSIs thus appear to have a key coordinating role within striatal networks, and there is a pressing need to better understand their physiological and behavioral functions. The proposed complementary experiments in brain slices and awake behaving animals make full use of advanced electrophysiological, pharmacological and optogenetic methods.
Aim 1 examines how distinct inputs from cortex, thalamus, and globus pallidus influence FSI firing patterns, both spontaneously and at critical moments of choice task performance.
Aim 2 examines the conditions under which FSIs control striatal projection cells of the two major output pathways, and how FSI suppression affects network dynamics and behavior. Finally, Aim 3 investigates the consequences of dopamine loss on striatal microcircuits, examining changes in local connectivity and firing patterns that may underlie core movement difficulties in Parkinson's Disease. The long-term goals of this research program are to determine the fundamental operational principles of striatal circuits from sub-cellular to network levels. This knowledge would be of immense value in designing improved therapies for Parkinson's Disease, dystonia, Tourette Syndrome and other serious brain disorders.
This proposal aims to reveal how microcircuitry within a specific brain structure contributes to normal behavioral control, and how dysfunction of this structure results in abnormal behavior. Greater understanding of this circuitry would be of immense value in designing improved therapies for Parkinson's Disease, dystonias, Tourette Syndrome and other serious brain disorders.
| Owen, Scott F; Berke, Joshua D; Kreitzer, Anatol C (2018) Fast-Spiking Interneurons Supply Feedforward Control of Bursting, Calcium, and Plasticity for Efficient Learning. Cell 172:683-695.e15 |
| Berke, Joshua D (2018) What does dopamine mean? Nat Neurosci 21:787-793 |
| Schmidt, Robert; Berke, Joshua D (2017) A Pause-then-Cancel model of stopping: evidence from basal ganglia neurophysiology. Philos Trans R Soc Lond B Biol Sci 372: |
| Howe, William M; Gritton, Howard J; Lusk, Nicholas A et al. (2017) Acetylcholine Release in Prefrontal Cortex Promotes Gamma Oscillations and Theta-Gamma Coupling during Cue Detection. J Neurosci 37:3215-3230 |
| Mirzaei, Amin; Kumar, Arvind; Leventhal, Daniel et al. (2017) Sensorimotor Processing in the Basal Ganglia Leads to Transient Beta Oscillations during Behavior. J Neurosci 37:11220-11232 |
| Roseberry, Thomas K; Lee, A Moses; Lalive, Arnaud L et al. (2016) Cell-Type-Specific Control of Brainstem Locomotor Circuits by Basal Ganglia. Cell 164:526-37 |
| Hamid, Arif A; Pettibone, Jeffrey R; Mabrouk, Omar S et al. (2016) Mesolimbic dopamine signals the value of work. Nat Neurosci 19:117-26 |
| Kharkwal, Geetika; Brami-Cherrier, Karen; Lizardi-Ortiz, José E et al. (2016) Parkinsonism Driven by Antipsychotics Originates from Dopaminergic Control of Striatal Cholinergic Interneurons. Neuron 91:67-78 |
| Parker, Philip R L; Lalive, Arnaud L; Kreitzer, Anatol C (2016) Pathway-Specific Remodeling of Thalamostriatal Synapses in Parkinsonian Mice. Neuron 89:734-40 |
| Mallet, Nicolas; Schmidt, Robert; Leventhal, Daniel et al. (2016) Arkypallidal Cells Send a Stop Signal to Striatum. Neuron 89:308-16 |
Showing the most recent 10 out of 26 publications
