Our long term goal is to provide a framework to understand how a simple, innate behavior emerges from the activity of the nervous system. For this, we will apply a battery of molecular genetics techniques and imaging technologies to study the circuit mechanisms underlying temperature preference in Drosophila -a system ideally suited for a comprehensive genetic and molecular dissection of neural circuits and behaviors. We have recently shown that temperature is represented by a map of activity created by sensory afferents at the first relay station in the fly brain. We now propose to study how this map is processed by central neural circuits and transformed into directed behavior. To accomplish our overall objectives, we will use a combination of targeted photo-activation of GFP, GRASP, ChR2-mediated circuit mapping and genetic expression of effector molecules to: 1) Identify ascending neuronal populations receiving temperature information and representing it to higher brain centers. 2) Study their tuning and properties, and narrow our search to the key pathways that mediate temperature navigation and preference. 3) Determine their connectivity to higher brain centers, study the transformation of stimuli at each station in the circuit and eventually track the descending pathways that control behavior. This work is expected to contribute to our general understanding of the wiring logic of the neural circuits that control innate behavior in animals, and potentially provide insights on how genetic conditions which affect wiring can result in compromised circuit function and altered behavior.

Public Health Relevance

Our long-term goal is to understand how sensory stimuli are represented in the brain and integrated to produce directed behaviors. The studies outlined here will provide insight into the neural basis of temperature processing and preference, and be useful in understanding the function of the nervous system in normal and disease state.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Project (R01)
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Molecular Neurogenetics Study Section (MNG)
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Gnadt, James W
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Northwestern University at Chicago
Schools of Arts and Sciences
United States
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Frank, Dominic D; Enjin, Anders; Jouandet, Genevieve C et al. (2017) Early Integration of Temperature and Humidity Stimuli in the Drosophila Brain. Curr Biol 27:2381-2388.e4
Arenas, Oscar M; Zaharieva, Emanuela E; Para, Alessia et al. (2017) Activation of planarian TRPA1 by reactive oxygen species reveals a conserved mechanism for animal nociception. Nat Neurosci 20:1686-1693
Enjin, Anders; Zaharieva, Emanuela E; Frank, Dominic D et al. (2016) Humidity Sensing in Drosophila. Curr Biol 26:1352-8
Macpherson, Lindsey J; Zaharieva, Emanuela E; Kearney, Patrick J et al. (2015) Dynamic labelling of neural connections in multiple colours by trans-synaptic fluorescence complementation. Nat Commun 6:10024
Frank, Dominic D; Jouandet, Genevieve C; Kearney, Patrick J et al. (2015) Temperature representation in the Drosophila brain. Nature 519:358-61