Myelinating cells, oligodendrocytes (OLs) in the CNS and Schwann cells (SCs) in the PNS, produce a vast amount of myelin sheath. Actively myelinating OLs and SCs must produce enormous amounts of myelin proteins to assemble myelin sheath during development. Mature OLs and SCs in adults need to produce a substantial amount of myelin proteins to maintain myelin structure homeostasis. Myelin proteins are synthesized, modified, and folded in the endoplasmic reticulum (ER). Maintaining ER protein homeostasis is essential and necessary for the function of myelinating cells. Currently available data indicate that actively myelinating OLs and SCs are more sensitive to disruption of ER protein homeostasis than mature OLs and SCs, due to the rate of myelin protein production. Nevertheless, the mechanisms by which myelinating cells maintain ER protein homeostasis remain unexplored. Misfolded/unfolded proteins in the ER are detected and degraded by a process known as ER-associated degradation (ERAD). In response to ER stress, activation of the unfolded protein response (UPR), which comprises three parallel signaling pathways PERK, IRE1, and ATF6?, restores ER protein homeostasis by facilitating protein folding, attenuating protein translation, and enhancing ERAD. Deletion of either PERK or ATF6? does not affect myelinating cells under normal conditions. Surprisingly, our preliminary results showed that double deletion of PERK and ATF6? in myelinating cells led to late-onset dysmyelination in the CNS of adult mice. Both PERK activation and ATF6? activation stimulates the expression of genes related to the Sel1L-Hrd1 complex, the best-characterized ERAD machinery. Intriguingly, our preliminary observation showed that Sel1L inactivation specifically in myelinating cells led to adult-onset dysmyelination in mice. Data indicate that overexpression of PLP, the most abundant myelin protein in the CNS and a substrate of ERAD, leads to soma retention of PLP in OLs, resulting in adult-onset dysmyelination in the CNS. Importantly, our preliminary data showed that both Sel1L inactivation and inactivation of PERK and ATF6? induced soma retention of PLP in OLs. Thus, in this proposal, we will test the hypothesis that the UPR is required for maintaining ER protein homeostasis and the viability and function of mature OLs in adults by regulating ERAD of PLP.
In Aim 1, we will demonstrate that inactivation of PERK and ATF6? impairs the viability and function of mature OLs in adult mice.
In Aim 2, we will demonstrate that Sel1L inactivation impairs the viability and function of mature OLs and SCs in adult mice.
In Aim 3, we will determine if Sel1L inactivation compromises the viability and function of mature OLs through impairment of ERAD of PLP and subsequent soma retention of PLP. This work will uncover a mechanism responsible for maintaining ER protein homeostasis in mature OLs. The knowledge gained from these studies will advance our understanding of the biology of myelinating cells.

Public Health Relevance

Myelin proteins are synthesized, modified, and folded in the endoplasmic reticulum, so maintaining endoplasmic reticulum protein homeostasis is essential and necessary for the function of myelinating cells. The goal of this project is to understand the roles of the unfolded protein response and endoplasmic reticulum- associated degradation in maintaining endoplasmic reticulum protein homeostasis in myelinating cells. These studies will provide novel insights into the mechanisms responsible for maintaining endoplasmic reticulum protein homeostasis in myelinating cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS105689-04
Application #
10115143
Study Section
Cellular and Molecular Biology of Glia Study Section (CMBG)
Program Officer
Morris, Jill A
Project Start
2018-03-15
Project End
2023-02-28
Budget Start
2021-03-01
Budget End
2022-02-28
Support Year
4
Fiscal Year
2021
Total Cost
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Neurosciences
Type
Schools of Medicine
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455