Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Project (R01)
Project #
5R01RR007855-06
Application #
2546589
Study Section
Special Emphasis Panel (ZRG7-SSS-3 (14))
Project Start
1992-09-30
Project End
1998-09-29
Budget Start
1997-09-30
Budget End
1998-09-29
Support Year
6
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Los Alamos National Lab
Department
Type
Schools of Arts and Sciences
DUNS #
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
Cui, H Helen; Valdez, Joseph G; Steinkamp, John A et al. (2003) Fluorescence lifetime-based discrimination and quantification of cellular DNA and RNA with phase-sensitive flow cytometry. Cytometry A 52:46-55
Crissman, H A; Steinkamp, J A (2001) Flow cytometric fluorescence lifetime measurements. Methods Cell Biol 63:131-48
Steinkamp, J A (2001) Time-resolved fluorescence measurements. Curr Protoc Cytom Chapter 1:Unit 1.15
Steinkamp, J A; Valdez, Y E; Lehnert, B E (2000) Flow cytometric, phase-resolved fluorescence measurement of propidium iodide uptake in macrophages containing phagocytized fluorescent microspheres. Cytometry 39:45-55
Keij, J F; Bell-Prince, C; Steinkamp, J A (2000) Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green, and MitoTracker Green is affected by mitochondrial membrane potential altering drugs. Cytometry 39:203-10
Keij, J F; Bell-Prince, C; Steinkamp, J A (1999) Simultaneous analysis of relative DNA and glutathione content in viable cells by phase-resolved flow cytometry. Cytometry 35:48-54
Steinkamp, J A; Lehnert, B E; Lehnert, N M (1999) Discrimination of damaged/dead cells by propidium iodide uptake in immunofluorescently labeled populations analyzed by phase-sensitive flow cytometry. J Immunol Methods 226:59-70
Steinkamp, J A; Lehnert, N M; Keij, J F et al. (1999) Enhanced immunofluorescence measurement resolution of surface antigens on highly autofluorescent, glutaraldehyde-fixed cells analyzed by phase-sensitive flow cytometry. Cytometry 37:275-83
Sailer, B L; Valdez, J G; Steinkamp, J A et al. (1998) Apoptosis induced with different cycle-perturbing agents produces differential changes in the fluorescence lifetime of DNA-bound ethidium bromide. Cytometry 31:208-16
Cobo, J M; Garcia-Canero, R; Valdez, J G et al. (1998) Attenuation of apoptotic DNA fragmentation by amiloride. J Cell Physiol 175:59-67

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