The long-term objective of this research is the development of a compact, robust, and user-friendly laser source for 2-photon- excited laser scanning microscopy. Femtosecond-duration pulses from a modelocked solid- state laser operating at wavelengths around 1.3 microns will be frequency- doubled to generate wavelength-tunable pulses in the wavelength range around 650 nanometers. The modelocked laser will be pumped by an array of laser diodes to reduce the size and cost of the source while improving its stability and reliability. Such a source will provide researchers in the biological and biophysical sciences much greater access to nonlinear laser microscopies while enhancing and expanding its capabilities. The source will be designed for compatibility with the large and growing base of laser scanning microscopes. The development of new and/or enhanced capabilities for microscopy will have a significant bearing on health. Improved means for visualizing submicron chemistry will aid the understanding of molecular processes in both normal and diseased states. Intracellular micropharmacology is one directly health-related example of nonlinear laser microscopy: Highly localized and specific treatments become possible by 2-photon uncaging of bioeffector molecules microscopy.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Project (R01)
Project #
1R01RR010075-01A1
Application #
2285470
Study Section
Special Emphasis Panel (ZRG7-SSS-1 (20))
Project Start
1995-08-01
Project End
1998-07-31
Budget Start
1995-08-01
Budget End
1996-07-31
Support Year
1
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Cornell University
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Ilday, F O; Buckley, J R; Lim, H et al. (2003) Generation of 50-fs, 5-nJ pulses at 1.03 micrometre from a wave-breaking-free fiber laser. Opt Lett 28:1365-7
Ilday, F O; Lim, H; Buckley, J R et al. (2003) Practical all-fiber source of high-power, 120-fs pulses at 1 micrometre. Opt Lett 28:1362-4
Lim, H; Ilday, F O; Wise, F W (2003) Generation of 2-nJ pulses from a femtosecond ytterbium fiber laser. Opt Lett 28:660-2
Liu, X; Beckwitt, K; Wise, F (2000) Transverse instability of optical spatiotemporal solitons in quadratic media. Phys Rev Lett 85:1871-4
Liu, X; Beckwitt, K; Wise, F (2000) Noncollinear generation of optical spatiotemporal solitons and application to ultrafast digital logic. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics 61:R4722-5