Abstract

These investigators have recently identified two closely related gammaherpesviruses present in retroperitoneal fibrosis lesions of immunosuppressed macaques. Preliminary data indicate that these newly discovered viruses (termed RFHV) are the simian counterparts of human herpesvirus 8, which is associated with Kaposi s sarcoma. In this application, the investigators propose to investigate the overall genomic structure of RFHV, to identify and characterize the RFHV homologs of cellular cytokine-like genes found in the HHV-8 genome, and to determine the prevalence of RFHV in retroperitoneal fibrosis lesions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Project (R01)
Project #
5R01RR013154-02
Application #
2797131
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Program Officer
Robinson, Jerry
Project Start
1997-09-30
Project End
2000-09-29
Budget Start
1998-09-30
Budget End
1999-09-29
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Pathology
Type
Schools of Public Health
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Howard, Kellie; Cherezova, Lidia; DeMaster, Laura K et al. (2017) ORF73 LANA homologs of RRV and MneRV2 contain an extended RGG/RG-rich nuclear and nucleolar localization signal that interacts directly with importin ?1 for non-classical nuclear import. Virology 511:152-164
Cherezova, Lidia; Burnside, Kellie L; Rose, Timothy M (2011) Conservation of complex nuclear localization signals utilizing classical and non-classical nuclear import pathways in LANA homologs of KSHV and RFHV. PLoS One 6:e18920
Bruce, A Gregory; Bakke, Angela M; Gravett, Courtney A et al. (2009) The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques. Virol J 6:205
Philipp-Staheli, Jeannette; Marquardt, Taya; Thouless, Margaret E et al. (2006) Genetic variability of the envelope gene of Type D simian retrovirus-2 (SRV-2) subtypes associated with SAIDS-related retroperitoneal fibromatosis in different macaque species. Virol J 3:11
Bruce, A Gregory; Bakke, Angela M; Bielefeldt-Ohmann, Helle et al. (2006) High levels of retroperitoneal fibromatosis (RF)-associated herpesvirus in RF lesions in macaques are associated with ORF73 LANA expression in spindleoid tumour cells. J Gen Virol 87:3529-38
Burnside, Kellie L; Ryan, Jonathan T; Bielefeldt-Ohmann, Helle et al. (2006) RFHVMn ORF73 is structurally related to the KSHV ORF73 latency-associated nuclear antigen (LANA) and is expressed in retroperitoneal fibromatosis (RF) tumor cells. Virology 354:103-15
Bielefeldt-Ohmann, H; Barouch, D H; Bakke, A M et al. (2005) Intestinal stromal tumors in a simian immunodeficiency virus-infected, simian retrovirus-2 negative rhesus macaque (Macaca mulatta). Vet Pathol 42:391-6
Bruce, A Gregory; Bakke, Angela M; Thouless, Margaret E et al. (2005) Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons. Virol J 2:2
Rose, Timothy M; Ryan, Jonathan T; Schultz, Emily R et al. (2003) Analysis of 4.3 kilobases of divergent locus B of macaque retroperitoneal fibromatosis-associated herpesvirus reveals a close similarity in gene sequence and genome organization to Kaposi's sarcoma-associated herpesvirus. J Virol 77:5084-97
Schultz, E R; Rankin Jr, G W; Blanc, M P et al. (2000) Characterization of two divergent lineages of macaque rhadinoviruses related to Kaposi's sarcoma-associated herpesvirus. J Virol 74:4919-28