Abstract

The proposed research offers an innovative approach for addressing serious errors encountered in in-vivo glucose monitoring through the rational design of oxygen-independent interference-free fluorocarbon/metalized-carbon enzyme electrodes. The use of perfluorochemicals, often employed as blood substitutes due to their remarkable oxygen solubility, as a major component of the biocomposite electrode, should satisfy the oxygen demand internally. The new catalytic metal-carbon component of the electrode would offer preferential detection of the enzymatically-liberated peroxide species, and freedom from common electroactive interferences. The resulting fluorocarbon/metalized-carbon biocomposite microelectrode will thus possess the high accuracy essential for triggering proper alarms, and for making valid therapeutic decisions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Project (R01)
Project #
5R01RR014173-02
Application #
6078312
Study Section
Special Emphasis Panel (ZRG7-SSS-8 (45))
Project Start
1998-09-30
Project End
2001-09-29
Budget Start
1999-09-30
Budget End
2000-09-29
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
New Mexico State University Las Cruces
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
City
Las Cruces
State
NM
Country
United States
Zip Code
88003

  Publications

Wang, Joseph; Chatrathi, Madhu Prakash (2003) Microfabricated electrophoresis chip for bioassay of renal markers. Anal Chem 75:525-9
Wang, Joseph; Li, Shifang; Mo, Jian-Wei et al. (2002) Oxygen-independent poly(dimethylsiloxane)-based carbon-paste glucose biosensors. Biosens Bioelectron 17:999-1003
Wang, J; Chatrathi, M P; Tian, B (2001) Microseparation chips for performing multienzymatic dehydrogenase/oxidase assays: simultaneous electrochemical measurement of ethanol and glucose. Anal Chem 73:1296-300
Wang, J; Chatrathi, M P; Ibanez, A (2001) Glucose biochip: dual analyte response in connection to two pre-column enzymatic reactions. Analyst 126:1203-6
Wang, J; Zhang, X (2001) Needle-type dual microsensor for the simultaneous monitoring of glucose and insulin. Anal Chem 73:844-7
Wang, J; Chatrathi, M P; Tian, B (2000) Micromachined separation chips with a precolumn reactor and end-column electrochemical detector. Anal Chem 72:5774-8
Wang, J; Chatrathi, M P; Tian, B et al. (2000) Microfabricated electrophoresis chips for simultaneous bioassays of glucose, uric acid, ascorbic acid, and acetaminophen. Anal Chem 72:2514-8
Wang, J; Chen, L; Hocevar, S B et al. (2000) One-step electropolymeric co-immobilization of glucose oxidase and heparin for amperometric biosensing of glucose. Analyst 125:1431-4
Wang, J (1999) Electroanalysis and biosensors. Anal Chem 71:328R-332R
Karyakin, A A; Vuki, M; Lukachova, L V et al. (1999) Processible polyaniline as an advanced potentiometric pH transducer. Application to biosensors. Anal Chem 71:2534-40

Showing the most recent 10 out of 11 publications